摘要
通过对复合酶超声辅助提取高寒香菊花多糖的工艺研究,以提取得率和纯度为指标,确定了适宜工艺条件。以未经酶解和未采用超声处理的多糖做对照,通过近红外光谱分析加酶及超声处理对高寒香菊花多糖结构的影响。结果表明:在复合酶2.5%(纤维素酶2.0%,酸性蛋白酶0.5%),酶解温度55℃,pH值4.5的条件下酶解2.0 h,多糖提取得率和纯度分别达到9.83%和28.58%,经聚酰胺柱纯化后纯度为64.57%。近红外光谱分析表明加酶和超声对多糖的结构没有显著影响,但提高了多糖提取得率。
With yield and purity of crude polysaccharide as evaluation indexes,the optimal extraction process conditions of polysaccharides from Coreopsis basalis flowers by ultrasoound-assisted compound enzymolysis was studied.Effect of enzymatic hydrolysis and ultrasonic treatment on the structure of Coreopsis basalis flowers polysaccharides was evaluated through the near infrared spectral analysis,no enzyme and ultrasound-assisted treatment was used as control.The results showed that the optimal extraction process conditions were： compound enzyme 2.5%（cellulase 2%,acid protease 0.5%）,temperature 55℃,pH 4.5,2.0 h.Under these conditions,the yield of crude polysaccharide was up to 9.83%,purity was 28.58%,and the purity was up to 64.57% after polyamide column purification.The result of near infrared spectral analysis showed that enzymatic hydrolysis and ultrasonic assisted extraction technology had no significant effect on the structure of Coreopsis basalis polysaccharides,but they can improve the extraction yield of polysaccharides.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第1期214-217,共4页
Food and Fermentation Industries
基金
科技型中小企业技术创新基金项目资助(10C26213200976)
关键词
复合酶
超声辅助提取
高寒香菊花多糖
光谱分析
compound enzyme
ultrasonic assisted extraction
Coreopsis basalis flower polysaccharide
spectroscopy analysis
