摘要
以根癌农杆菌(Agrobacterrium tumefaciens)介导的紫色红曲菌(Monascus purpureus)突变体库中的538株转化子菌株为材料,通过菌落形态观察并结合显微特征分析,筛选获得了9株形态特征变化显著的转化子,对其进行了分子验证和遗传稳定性检测,证明转化子基因组DNA中含有T-DNA插入片段,并能稳定遗传.在此基础上,采用乙醇提取法和改良的纸色谱法分别对转化子的主要代谢产物红曲色素和γ-氨基丁酸(γ-Ami-nobutyric Acid,GABA)进行了分析,发现突变株产生次级代谢产物的能力有不同程度的变化.
538 transformants from T-DNA mutant library of Monascus purpureus mediated by Agrobacterrium tumefaciens were used as materials, 9 respresentative transformants with significant changes were selected through the colony and mirco-morphological observation, and verified by PCR amplification. The results showed that there were T-DNA insertions in transformants genome. Through stability examination, all 9 respresentative transformants could be steadily inherited. Based on this, two main metabolites of 9 respresentative transformants, which were pigments and T-aminobutyrie acid （ GABA ） , were analyzed respectively by using the methods of ethanol extraction and paper chromatography. The results showed that the capacity of producing metabolites by transformants changed in different degrees compared with the original strain. It provided the foundation for the regulation mechanism of Monascus metabolite research.
出处
《浙江师范大学学报（自然科学版）》
CAS
2012年第1期104-109,共6页
Journal of Zhejiang Normal University:Natural Sciences
基金
国家自然科学基金资助项目(31070008)
浙江省自然科学基金资助项目(Y3090343)
2011年浙江省大学生科技创新活动计划资助项目(2011R404011)
关键词
红曲菌
突变体库
转化子
红曲色素
γ-氨基丁酸
Monascus purpureus
mutant library
transformants
pigment
T-aminobutyric acid （ GABA ）
