摘要
目的了解6类抗生素耐药基因在耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)中的分布情况,为抗生素的合理使用及感染控制提供依据。方法应用K-B纸片扩散法对6种抗生素进行药敏试验,聚合酶链反应(PCR)对6类耐药基因进行检测。结果 MRCNS对氨苄西林、红霉素、四环素、庆大霉素和莫匹罗星的耐药率分别为100%、87.50%、30.21%、61.46%和27.08%,未检测出对万古霉素的耐药菌株;mecA、ermB、ermC、msrA、tetM、tetK、aac(6’)/aph(2")、aph(3’)-III和mupA耐药基因的阳性检出率分别为100.0%、10.42%、77.08%、46.86%、5.21%、2.08%、75.00%、39.58%和25.00%,未检测出ermA、ant(6’)-I、vanA和vanB耐药基因。结论 MRCNS能同时携带多种抗生素耐药基因,为多重耐药菌。
Objective To study the distribution of 6 kinds of antibiotic resistance genes in methicillin-resistant coagulase negative Staphylococcus（MRCNS）,in order to guide the rational use of antibiotics and infection control.Method K-B disk diffusion test was used for determinating the susceptibility to 6 kinds of antibiotics;polymerase chain reaction（PCR） was used for determinating the 6 kinds of resistance genes.Result The resistance rates to ampicillin,erythromycin,tetracycline,gentamicin and mupirocin of MRCNS were 100%,87.50%,30.21%,61.46% and 27.08%,respectively;Vancomycin-resistant strains were not found;the test of resistance genes showed that the occurrence of mecA,ermB,ermC,msrA,tetM,tetK,aac（6＇）/aph（2＂）,aph（3＇）-III and mupA was 100.0%,10.42%,77.08%,46.86%,5.21%,2.08%,75.00%,39.58% and 25.00%,respectively,while the resistance genes of ermA,ant（6＇）-I,vanA and vanB were negative.Conclusion MRCNS can simultaneously carry multiple antibiotic resistance genes and be multiple drug resistant.
出处
《中国微生态学杂志》
CAS
CSCD
2012年第2期142-144,148,共4页
Chinese Journal of Microecology
基金
温州市鹿城区科技计划项目(S090210)
关键词
凝固酶阴性葡萄球菌
甲氧西林耐药
耐药基因
Coagulase negative staphylococcus
Methicillin resistant
Drug resistant gene
