摘要
利用化学共沉淀方法,制得了主要成分为Fe3O4的超顺磁性纳米磁珠。纳米磁珠和硅烷偶联剂氨丙基三乙氧基硅烷(APTES)反应而带上氨基基团。带上氨基的磁珠通过戊二醛活化后发生醛基化,与黄曲霉毒素B1(AFB1)多克隆抗体偶联得到黄曲霉毒素B1免疫磁珠。利用该免疫磁珠为净化工具,建立了有机溶剂萃取、免疫磁珠净化、高效液相色谱(HPLC)检测植物油中黄曲霉毒素B1的方法。该方法具有良好线性,线性范围为5～50μg/L,相关系数达0.999 4,检出限为0.5μg/L,平均回收率为96%,相对标准偏差为12.5%。该方法操作简单、灵敏度高、准确性好,可用于植物油中黄曲霉毒素B1的检测。
Using the method of chemical co-precipitation, the super paramagnetic nanoparticles with the key component of Fe3O4 were synthesized. The magnetic beads reacted with the silane coupling agent 3-aminopropyl triethoxysilane and connected with amino groups. After activated by the agent glutaraldehyde, the magnetic beads took along the aldehyde group and coupled with aflatoxin B1 polyclonal antibody to form the immune magnetic beads. A method was established to detect the aflatoxin B1 in vegetable oil by using the organic solvent for preliminary extraction, the aflatoxin B1 immune magnetic beads system to purification and HPLC to quantification. The method showed a good linearity in the range of 5 - 50 μg/L with correlation coefficient of 0. 999 4 and detection limit of 0. 5 μg/L. The average recovery was up to 96% and the RSD was 12.5%. With the advantages of simplicity, sensitivity and high accuracy, the method could be applied in the determination of aflatoxin B1 in vegetable oil.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第12期1345-1350,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(20806033)
张家港市科技局资助项目(ZKS0904)
