摘要
建立了植物油中苯并(a)芘的分子印迹固相萃取小柱-高效液相色谱快速测定方法。植物油样品经正己烷溶解后,转移至活化和平衡后的分子印迹固相萃取小柱,正己烷淋洗,二氯甲烷洗脱,氮吹至近干后,1mL乙腈溶解,0.22μm有机系滤膜过滤,高效液相色谱荧光检测器测定,保留时间定性,色谱峰面积定量。苯并(a)芘在0.5～20.0 ng/mL范围内呈良好线性,线性相关系数为0.999,检出限均为0.2μg/kg。0.5,2.0,10.0μg/kg 3个不同添加水平的回收率为90.6%～102%,相对标准偏差(RSD)分别小于5.0%。该方法具有简便、快速、灵敏高、准确度好等优点,可满足植物油中苯并(a)芘的定量分析。
A rapid molecularly imprinted solid phase extraction HPLC method of benzo(a)pyrene in vegetable oil was established. Prepared samples were dissolved with n-hexane. The disposed samples were transferred to activated and balanced molecularly imprinted solid phase extraction microcolumn,washed with n-hexane,eluated with dichloromethane,dried with nitrogen,dissolved with 1mL acetonitrile,screened with 0.22 μm organic filter membrane. Then,they were detected by HPLC fluorescence detector,qualitatively analyzed with retention time,quantitatively analyzed with peak area. Benzo (a)pyrene showed a good linear relationship at a range of 0.5 to 20.0 ng/m,with the limit of determination of 0.2 μg/ kg, correlation coefficients of benzo(a)pyrene was 0.999. Tests for recovery and precision in 0.5,2.0,10.0 μg/kg three adding levels,giving results of average recovery of 90.6% to 102% and relative standard deviation under 5.0%. The established method is simple,rapid,sensitive and accurate. It can be used to determine benzo(a)pyrene accurately in vegetable oil.
作者
许蓓蓓
王振涛
程晓宏
陈彬
杨俊
周楠
Xu Bei-bei;Wang Zhen-tao;Cheng Xiao-hong1;Chen Bin;Yang Jun;Zhou Nan(Nantong Center for Food and Drug Control,Nantong,Jiangsu 226006,China;Nantong Product Ouality Snpervision and Institate,Natong,Jiangsu 226011,China)
出处
《福建分析测试》
CAS
2018年第5期30-33,共4页
Fujian Analysis & Testing
基金
江苏省南通市科技局食品安全专项(MS12017018-3)
应用基础研究(YYZ17098)
江苏省食品药品监督管理局科研项目(20170225)
