摘要
黑曲霉菌株D-597所产α-葡萄糖苷酶是胞内酶,完整细胞呈现的酶活力较低。研究了吐温-80、十六烷基溴化铵(CTAB)、乙醚、丙酮、戊二醛、乙醇等渗透剂的透性化效果,并对最适渗透剂戊二醛的透性化条件进行了优化,最优透性化处理条件为:每1g湿菌丝体添加30mL浓度为10%(v/v)的戊二醛溶液,处理温度30℃,处理时间60min。制备所得的透性化细胞的表观酶活达到483.9U/g湿菌体,是完整细胞表观酶活的193.3%。使用透性化细胞转化生产低聚异麦芽糖(IMO),其转化周期为24h,较完整细胞缩短24h,转化率保持在70%以上,具有一定的工业化应用前景。
α-glucosidase was an intracellular enzyme in Aspergillus niger. The whole cells of Aspergillus niger exhibited low transglucosylation activity. Various chemical agents such as tween-80,cetyltrimethylammoinium bromide,diethyl ether,acetone,glutaral and ethanol were used to permeabilize the mycelium and demonstrated that glutaral was the most effective of them. The maximum transglucosylation activity（483. 9U/g wet cells,increased by 93. 3% compared to the whole cells）was obtained when cells were treated with 30mL 10%（v/v）glutaral per gram wet cells at 30℃ for 60min. The permeabilized Aspergillus niger cells convert liquefied cassava starch to isomaltooligosaccharides（IMO）within 24h（shorten by 24h than the whole cells）,the IMO yield accounted for 70%,demonstrating the research had great potential on IMO industrial production.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第12期72-74,77,共4页
Science and Technology of Food Industry
基金
国家自然科学基金项目(20362001)
国家"863"项目(2006AA10Z339)
关键词
黑曲霉
低聚异麦芽糖
透性化
Α-葡萄糖苷酶
Aspergillus niger
isomaltooligosaccharides
permeabilization
alpha-glucosidase
