摘要
目的分析c-kit和缝隙连接蛋白43(Cx43)在新生儿自发性胃穿孔(spontaneous neonatal gastricperforation,SNGP)中的表达情况,探讨Cajal间质细胞(interstitial cell of Cajal,ICC)在新生儿SNGP中的意义。方法收集2001～2010年手术治疗的19例SNGP胃组织标本,以8例无消化道畸形新生儿尸检胃组织作为对照,采用免疫组织化学SP法进行c-kit和Cx43检测。结果 SNGP患儿胃壁穿孔处肌层缺失,穿孔周围肌束明显变薄。与对照组比较,SNGP患儿胃组织中c-kit阳性细胞以及Cx43阳性细胞表达明显减少(P<0.01)。结论新生儿SNGP与胃壁ICC数量减少、缝隙连接结构破坏有关。
Objective To study the clinical significance of interstitial cell of Cajal（ICC） in spontaneous neonatal gastric perforation by examining the expression of c-kit and Cx43 in neonates with this disorder.Methods The gastric specimens of 19 cases of neonatal gastric perforation from 2001 to 2010 and 8 cases of accidental death without digestive tract malformations（control） were collected.Immunohistochemical staining was employed to examine the expression of c-kit and Cx43（immunomarkers of ICCs） in gastric tissues.Results The muscular layer of the stomach wall became thinner or deficient in the gastric perforation group.C-kit and Cx43 positive cells in gastric tissues decreased significantly in the gastric perforation group compared with those in the control group（P0.01）.Conclusions The development of spontaneous neonatal gastric perforation is associated with the decreased quantity of ICCs and damaged gap junction structure of the stomach wall.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2011年第10期787-789,共3页
Chinese Journal of Contemporary Pediatrics
