摘要
目的:探讨酪氨酸激酶膜受体基因(C-kit)、干细胞因子(SCF)与缝隙连接蛋白(Cx43)在先天性巨结肠发病机制中的作用。方法:收集68例先天性巨结肠(HD)患儿手术切除标本的存档蜡块,根据HE染色结果,将神经节细胞正常的扩张段标本设为扩张组,无神经节细胞的狭窄段标本设为狭窄组。并收集8例无消化道畸形新生儿尸检结肠组织作为对照。用免疫组织化学EliVision法检测C-kit、SCF及Cx43蛋白的表达,原位杂交法检测C-kit mRNA的表达。结果:与对照组比较,HD扩张组中C-kit、SCF、Cx43的表达差异无统计学意义(P>0.05),狭窄组中C-kit、SCF、Cx43的表达差异有统计学意义(P<0.05)。结论:先天性巨结肠的发生与C-kit、SCF及Cx43表达减少有关。
Objective： To explore the action of C-kit, SCF and Cx43 in the pathogenesis of HD by examining the expression and distribution of C-kit, SCF and Cx43. Methods： The colon specimens of 68 cases of HD and 8 cases of accidental death without digestive tract malformations （control） were collected. The case group composed of a expansion group in which the ganglion cells were normal in the intestinal wall and a narrow group in which the ganglion cells were absent in the intestinal wall according to diagnosis by HE. Immunohistochemical staining was used to detect the proteins of C-Kit, SCF and Cx43, and hybridization in situ to detect the mRNA of C-Kit in all specimens. Results： Expression of C-kit, SCF and Cx43 in the expansion group was not significantly different from the control group （P 〉 0.05）, while expression of C-kit, SCF and Cx43 in narrow group decreased significantly compared with the control group （P 〈 0.05）. Conclusion： The development of Hirschsprung＇s disease is associ- ated with the decreased expression of C-kit, SCF and Cx43.
出处
《温州医学院学报》
CAS
2013年第5期319-322,共4页
Journal of Wenzhou Medical College
