摘要
目的研究Cajal间质细胞、nNOS、ccSMA在胃食管反流（GER）动物模型的表达，探讨GER发生的机制及病理改变。方法采用幼兔建立GER动物模型，应用免疫组织化学、RT-PCR等方法探讨GER动物模型ICC、nNOS及盯SMA的形态特点及分布规律。结果GER组ICC形态异常，突起变短、钝，弱阳性、阴性染色，阳性强度均值、阳性区域面积减少；NOS阳性产物增多、染色增强，强阳性、阳性染色，阳性强度均值、阳性区域面积显著增多，差异有显著性意义；盯SMA与对照组比较阳性强度均值、阳性区域面积变化不明显。RT-PCR示c-kit基因扩增后获得的DNA片段245bp，GER组基因表达明显下降；nNOS基因的DNA片段602bp，GER组基因表达明显增高，SMA基因的DNA片段239bp，GER组、对照组基因表达变化不明显。结论ICC、nNOS的病理变化与GER疾病发生有密切关系，ICC、nNOS和SMA相互影响，相互作用，一起参与了GER的病理生理过程。
Objective To investigate the expressions of ICC, nNOS, and α-SMA in GER experimental animal model. Methods In this experiment, we establish the GER animal model with the rabbits, applying the immunhistological measurement and RT-PCR method to clarify the expressions of ICC, nNOS, and α-SMA in GER animal model. Results ICC in normal rabbits are found throughout the distal esophagus, the body and antrum of stomach interspersed within the circular and longitudinal muscle layers and also forming a dense network at the level of the neuronal myenteric plexus. While in GER animals, the area and degree of ICC-positive staining were significantly less than those of controls. ICC had fusiform cell body with two longer processes morphologically. NOS distributed in neuronal myenteric plexus of esophagus, the body and antrum of stomach. The shape of NOS positive neurons was basically similar, most of them being round and oval in shape. The size and staining intensity of NOS positive neurons in GER rabbits were larger than those in controls, α-SMA positive staining was nearly absent in both control and GER animals. RT-PCR analysis showed that decreased expression of c-kit, increased NOS and similar SMA expression in GER rabbits compared with those of controls. Conclusions The impaired interaction between ICC, NOS and SMA may be contributed to the development of GER in this animal model of GER.
出处
《中华小儿外科杂志》
CSCD
北大核心
2007年第2期82-86,共5页
Chinese Journal of Pediatric Surgery
