摘要
目的:对VEGF-D在NSCLC肺癌组织及淋巴结组织中的表达进行研究,探讨VEGF-D在NSCLC淋巴管生成和淋巴结早期转移中的作用。方法:收集非小细胞肺癌患者术后肺组织标本52例,采用半定量RT-PCR的方法对NSCLC肺癌组织中及淋巴结组织中的VEGF-D mRNA的表达进行检测。结果:(1)VEGF-D mRNA在淋巴结转移阳性组的阳性淋巴结组织中的表达水平较淋巴结转移阴性组中阴性淋巴结中的表达水平降低(P<0.05)。(2)在淋巴结转移阳性组中VEGF-D mRNA在阳性淋巴结与阴性淋巴结中的表达水平无显著区别(P>0.05)。结论:VEGF-D mRNA的表达水平与肺癌淋巴结转移有显著的相关性,在预测肺癌的淋巴结转移,完善分子分期,指导临床治疗上有重要意义。
Objective： vascular endothelial growth factor （VEGF）-D is supposed to participate in lymphangiogenesis. The purpose of this study was to clarify the clinical significance of the expression of the factor and to evaluate its relationship with lymph node metastasis in patients with non-small cell lung cancer. Methods： Lung tissues specimen were collected from 52 NSCLC patients who underwent operation. A semiquantitative multiplex RT-PCR tec^hnology was applied to measure the mRNA quantity of VEGF-D in carcinoma and lymph node tissue; Result：（1） The VEGF-D mRNA level in lung tumor tissue was lower in lymph node positive group than that in lymph node negtive group （P〈0.05）; （2）in lymph node positive group, there was no different of VEGF-D mRNA level in positive lymph node and negative lymph node. Conclusion： The result of the study indicate that VEGF-D expression levels can predict lymph node metastasis. They can be a useful predictor of lymph node metastasis in NSCLC.
出处
《中国医药导刊》
2011年第9期1510-1512,共3页
Chinese Journal of Medicinal Guide
