摘要
背景与目的:血管内皮生长因子C(vascularendothelialgrowthfactor-C,VEGF-C)和D(vascularendothelialgrowthfactor-D,VEGF-D)是目前已鉴定出的淋巴管生长因子,有研究表明肿瘤组织中VEGF-C或VEGF-D过表达与淋巴转移有关。本研究旨在探讨鼻咽癌组织中VEGF-C和VEGF-D的表达情况及其临床意义。方法:采用免疫组化SP法检测66例鼻咽癌组织中VEGF-C和VEGF-D的表达情况,同时检测血管内皮生长因子受体3(vascularendothelialgrowthfactorreceptor-3,VEGFR-3)和CD34染色情况并计数微淋巴管密度(lymphaticmicrovesseldensity,LMVD)和微血管密度(microvesseldensity,MVD)。结果:VEGF-C高表达率在鼻咽癌组织中(54.5%)较鼻咽非肿瘤组织中(26.3%)高(P<0.05);鼻咽癌伴区域淋巴结转移或T分期晚者,VEGF-C高表达率增高,单因素及多因素Logistic回归分析均表明区域淋巴结转移与VEGF-C高表达相关(P<0.05),但VEGF-C高表达与性别、年龄、5年生存率、LMVD、MVD等因素无关(P>0.05)。VEGF-D阳性表达率在鼻咽癌组织中(69.7%)较鼻咽非肿瘤组织中(42.1%)高(P<0.05);鼻咽癌中VEGF-D阳性表达与性别、年龄、T分期、区域淋巴结转移、LMVD、MVD等因素无关(P>0.05),但与VEGF-C高表达显著正相关(P<0.01),VEGF-D阳性表达者5年生存率(50.0%)显著低于VEGF-D不表达者(85.0%)(P<0.01)。结论:鼻咽癌中VEGF-C高表达与区域淋巴结转移密切相关;VEGF-D阳性表达与区域淋巴结转移无关,但与VEGF-C高表达正相关,且与5年生存率密切相关。
BACKGROUND ＆ OBJECTIVE： Recently, vascular endothelial growth factor-C （VEGF-C） and -D （VEGF-D） have been identified as specific lymphangiogenic factors, and their overexpression are related to lymphatic metastasis. This study was to investigate the expression and clinical significance of VEGF-C and VEGF-D in nasopharyngeal carcinoma （NPC）. METHODS： The expression of VEGF-C, VEGF-D, vascular endothelial growth factor receptor-3 （VEGFR-3）, and CD34 in 66 specimens of NPC and 19 specimens of nasopharyngitis tissue were detected by SP immunohistochemistry. Lymphatic microvessel density （LMVD） and microvessel density （MVD） were calculated. RESULTS, The high expression rate of VEGF-C was significantly higher in NPC than in nasopharyngitis tissues （54.5% vs. 26.3%, P〈0.05）. The high expression rate of VEGF-C was significantly higher in the NPC with regional lymph node metastasis than in those without, and higher in the NPC with high T stage than in those with low T stage （P〈0.05）. Both univariate and multivariate logistic regression analyses showed that high expression of VEGF-C was correlated to regional lymph node metastasis （P〈0.05）, but not to age, sex, 5-year survival, LMVD, and MVD （P〉0.05）. The positive rate of VEGF-D was significantly higher in NPC tissues than in adjacent non-cancerous tissues （69.7% vs. 42.1%, P〈0.05）. In NPC tissues, VEGF-D expression had no correlations to age, sex, T stage, regional lymph node metastasis, LMVD, and MVD （P〉 0.05）, but was positively related to high expression of VEGF-C. The 5-year survival rate was significantly lower in VEGF-D-positive NPC than in VEGF-D- negative NPC （50.0% vs. 85.0%, P〈0.05）. CONCLUSION, In NPC, high expression of VEGF-C is closely correlated to regional lymph node metastasis; positive expression of VEGF-D shows no correlation to regional lymph node metastasis, but is positively related to high expression of VEGF- C and closely correlated to 5-year survival rate.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2007年第1期90-95,共6页
Chinese Journal of Cancer
关键词
VEGF—C
VEGF—D
鼻咽肿瘤
免疫组化
HE染色
微淋巴管密度
微血管密度
生存率
Vascular endothelial growth factor C （VEGF-C）
VEGF-D
Nasopharyngeal neoplasm
Immunohistochemistry
Lymphatic microvessel density
Microvessel density
Survival rate
