摘要
目的了解临床分离的产超广谱β-内酰胺酶(ESBLs)的肺炎克雷伯菌的耐药性及氨基糖苷类修饰酶基因的存在状况。方法对临床分离的35株产ESBLs肺炎克雷伯菌采用聚合酶链反应技术(PCR)检测6种氨基糖苷类修饰酶aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6')-Ⅰ,aac(6')-Ⅱ,ant(3")-Ⅰ,ant(2")-Ⅰ。结果该35株肺炎克雷伯菌检出:aac(3)-Ⅰ阳性4株(11.8%),aac(3)-Ⅱ阳性7株(20.6%),aac(6')-Ⅰ阳性13株(37.1%),aac(6')-Ⅱ阳性7株(20%),ant(3")-Ⅰ阳性11株(31.4%),ant(2")-Ⅰ阳性12株(34.3%)。共有26株(74.3%)检出AMEs。同一菌株可产生两种或两种以上的氨基糖苷类修饰酶基因。结论临床分离的产ESBLs肺炎克雷伯菌氨基糖苷类修饰酶基因携带率高。
Objective To study the antimicrobial resistance and genotyping ofaminoglycosides-modifying enzymes （AMEs） in ESBLs-producing Klebsiella preumoniae （KPN） isolated from hosipital-acquired infection. Methods Six A/VIEs genotypes of KPN were verified by polymerase chain reaction （PCR）. Results PCR shows that ： 26 strains of KPN （74.3%） could be found one or more type of AMEs gene.The positive rate of six AMEs gene： aac（3）- Ⅰ ,aac（3）- Ⅱ ,aac（6＇）- Ⅰ ,aac （6＇）- Ⅱ ,ant（3＂） - Ⅰ ,ant（2＂）- Ⅰ were 11.8%, 20.6%,37.1%, 20%,31.4%,34.3%. Two or more AMEs were produced by the same bacilli. Conclusion There is high positive percentages of AMEs gene in the ESBLs-producing Klebsiella pneumoniae.
出处
《中国现代药物应用》
2009年第15期5-6,共2页
Chinese Journal of Modern Drug Application
关键词
肺炎克雷伯菌
氨基糖苷类修饰酶
基因
Klebsiella pneumoniae
aminoglycoside modifying enzymes
gene
