摘要
目的研究金雀异黄素(genistein,Gen)对类风湿关节炎(rheumatoid arthritis,RA)动物模型胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)MAPK信号传导通路的影响。方法建立CIA大鼠模型,将培养的CIA大鼠FLS采用随机法分组,采用四甲基偶氮唑盐(MTT)法检测Gen(浓度分别为50、100、200μmol/L)对CIA大鼠FLS增殖的影响;采用免疫印迹(Western blot)法检测Gen(浓度分别为50、100、200μmol/L)对CIA大鼠FLS细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)和磷酸化的细胞外信号调节激酶(phosphorylated extracellular signal-regulated kinase,p-ERK)表达的影响。结果 Gen能够抑制CIA大鼠FLS的增殖,Gen高剂量组细胞增殖度在72h仅为1.10±0.04,明显低于模型组2.12±0.03(P<0.01),并且Gen作用于FLS细胞后,p-ERK表达降低,Gen高剂量组仅为0.34±0.02,明显低于模型组2.68±0.14(P<0.01),而ERK表达无改变(P>0.05)。结论 Gen能够抑制CIA大鼠FLS的增殖,其作用机制主要与下调MAPK信号传导通路的酪氨酸激酶,抑制ERK的磷酸化有关。
Objective To study the effect of genistein（Gen） on MAPK signal pathway in the CIA rat fibroblast-like synoviocytes（FLS）.Methods The rat model of collagen-induced arthritis（CIA） was established.The cultured FLS of CIA rats were divided using randomized method.The effects of Gen（at the concentration of 50,100,and 200 μmol/L,respectively） on the proliferation of FLS in CIA rats using methyl thiazolyl tetrazolium（MTT） assay.Effects of Gen（at the concentration of 50,100,and 200 μmol/L,respectively） on the expressions of extracellular signal-regulated kinase（ERK） and phosphorylated extracellular signal-regulated kinase（p-ERK） in the FLS of CIA rats were detected.Results Gen could inhibit the proliferation of FLS in CIA rats.The FLS proliferation in the high dose Gen group at 72 h was only 1.10± 0.04,significantly lower than that in the model group（2.12±0.03,P0.01）.Besides,after Gen＇s action on FLS,the expression of p-ERK was down-regulated.It was only 0.34±0.02 in the high dose Gen group,significantly lower than that in the model group（2.68±0.14,P0.01）.There was no change in the expression of ERK（P0.05）.Conclusions Gen could inhibit the proliferation of FLS in CIA rats.Its mechanism of action was mainly correlated to down-regulating the tyrosine kinase of MAPK signal transduction pathway and inhibiting phosphorylation of ERK.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2011年第10期1405-1408,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
江苏省高校自然科学基础研究项目(No.07KJD320245)
关键词
金雀异黄素
胶原诱导性关节炎
细胞外信号调节酶/磷酸化的细胞信号外节酶
成纤维样滑膜细胞
genistein
collagen-induced arthritis
collagen-induced arthritis extracellular signal-regulated kinase/phosphorylated extracellular signal-regulated kinase
fibroblast-like synoviocyte
