摘要
目的对青海省新发现的HIV-1抗体阳性的感染者进行首次CD4+T淋巴细胞绝对值进行测定,了解其免疫状况及疾病进展状况。方法对采集的168份全血样本以四色荧光抗体进行标记,用流式细胞仪进行CD4+T淋巴细胞绝对数的检测,统计分析其测定值。结果 168例HIV感染者的CD4+T淋巴细胞均数为(323.92±198.66)个/μL,CD4+T淋巴细胞计数≤200个/μL的占31.55%,201～350个/μL的占29.17%,351～500个/μL的占23.81%,>500个/μL的占15.48%。不同性别间CD4+T淋巴细胞均值的差异无统计学意义(t=1.67,P>0.05);4种不同感染途径的CD4+T淋巴细胞均值的差异有统计学意义(F=3.52,P<0.05),同性性传播组的数值明显高于输血液组;不同年龄组间CD4+T淋巴细胞均值的差异有统计学意义(F=7.52,P<0.05),18～30岁年龄组的数值最高。结论青海省HIV感染者的CD4+T淋巴细胞免疫水平普遍偏低,60.71%感染者已进入艾滋病期,应对其定期进行CD4+T淋巴细胞检测,监测疾病进展和及时给予抗病毒治疗,以把握治疗的最佳时期。
Objective To detect the absolute value of CD4＋T lymphocytes in newfound HIV-1 antibody positive patients of Qinghai province and understand its immune status and progression of disease.Method 168 samples of whole blood were collected,which marked by tetrachromatism fluorescent antibody,and then using flow cytometer to detect the absolute value of CD4＋T lymphocytes and statistical analysis the measured data.Results The CD4＋T lymphocytes mean of 168 cases were 323.92±198.66 / u l,among them,less than 200/ul was accounted for 31.55%,from 201 to 350/ul was accounted for 29.17%,and from 351 to 500/ul was 23.81%,more than 500/ul was 15.48%.There were no statistical significance between difference gender.（t = 1.67,P 0.05）.CD4＋T lymphocytes mean of 4 different infection route had statistical significance（F=3.52,P0.05）,gay sex group was higher than the blood transfusion group.Mean between difference age had statistical significance（F=7.52,P 0.05）,18 to 30 years old group was the highest.Conclusions CD4＋T lymphocytes immune level of HIV persons was low in Qinghai province,and 60.71% of infected person had entered into the AIDS phase,so regular CD4＋T lymphocytes detection need to carry out,we also need to monitoring the disease progression and supply antiviral treatment in time and grasp the best period of treatment.
出处
《医学动物防制》
2011年第10期904-906,共3页
Journal of Medical Pest Control
