摘要
目的:考察鲜香附挥发油的镇痛活性,并分析其化学成分。方法:挥发油提取采用水蒸气蒸馏;镇痛活性采用小鼠醋酸扭体法;成分分析采用GC-MS法。结果:鲜香附挥发油含量达0.4%;挥发油(0.1 g/kg)能够显著减少醋酸所致小鼠扭体次数;GC-MS方法鉴定出54个化学成分,占总挥发油的85.1%,其中主要含香附子烯(41.03%)、氧化石竹烯(5.32%)、α-芹子烯(4.37%)、α-可巴烯(4.36%)、naphthalene,6-isoproenyl-4,8a-dimethyl-1,2,3,5,6,7,8,8a-octahydro-(3.80%)、α-香附酮(3.11%)。结论:鲜香附挥发油具有良好镇痛效果,挥发油化学结构多样,相对含量高于1%的峰占总挥发油的66.4%,其中,香附子烯相对百分含量最高。
Objective：To observe the analgesic effect and analysis the chemical components of essential oil（EOC） from fresh rhizome of Cyperus rotundus.Methods：Hydrodistillation method was used to extract EOC;Acetic acid induced mice stretching model was applied to evaluate the analgesic effect of EOC.Gas chromatography coupled with mass spectrometry（GC-MS） were applied for the analysis of EOC.Results：The yield of EOC was 0.4%.The stretching times of mice induced by acetic acid were significantly reduced by EOC at dosage of 0.1 g/kg.54 ingredients,85.1% of which were EOC were identified by GC-MS analysis;The major compounds were cyperene（41.03 %）,β-caryophyllene oxide（5.32%）,α-selinene（4.37%）,α-copaene（4.36%）,naphthalene,6-isoproenyl-4,8a-dimethyl-1,2,3,5,6,7,8,8a-octahydro-（3.80%）and α-cyperone（3.11%）.Conclusion： The essential oil from fresh rhizome of Cyperus rotundus shows structure diversity and good analgesic effect.
出处
《中药材》
CAS
CSCD
北大核心
2011年第8期1225-1229,共5页
Journal of Chinese Medicinal Materials
基金
上海交通大学"第三期国家大学生创新性实验计划"(091024848)
综合性新药研究开发技术大平台(2009ZX09302-007)
关键词
鲜香附
挥发油
镇痛
GC-MS
Fresh rhizome of Cyperus rotundus
Essential oil
Analgesic activity
GC-MS analysis
