摘要
目的:建立肠肽胶囊中三七有效成分的HPLC含量测定方法。方法:色谱柱为SinoChrom ODS-BP(150 mm×4.6mm,5μm),以乙腈(A)和水(B)为流动相,梯度洗脱,检测波长为203 nm,流速为1.0 ml·min^(-1),柱温为室温,进样量为20μl。结果:三七皂苷R_1、人参皂苷Rg_1及人参皂苷Rb_1的线性范围依次为63.9～319.5μg·ml^(-1)、112.6～563.2μg·ml^(-1)、102.5～512.8μg·ml^(-1),线性关系良好(r值分别为0.999 7、0.999 4、0.999 9);三种皂苷的平均回收率分别为96.47%、98.75%,97.55%,RSD分别为1.52%、1.73%、1.81%(n=6)。结论:本方法简便可行、准确、灵敏度高、专属性强,可用于肠肽胶囊的质量控制。
Objective： To develop an HPLC method for the simultaneous determination of active ingredients in Changtai capsules. Method： The HPLC method was carried out on a SinoChrom ODS-BP column （ 150 mm × 4. 6 mm,5 μm） at room temperature. The mobile phase consisted of acetonitrile （A）-water （B） with gradient elution, the flow rate was 1.0 ml·min^-1, the UV detection wavelength was at 203 nm and the injection volume was 20 μl. Result： The calibration curves showed good linear correlation within the range of 63.9-319. 5 μg·ml^-1 for Notoginsenoside R1 （r =0. 999 7） ,112. 6-563.2 μg·ml^-1 for Ginsenoside Rg1 （r =0. 999 4） and 102. 5-512. 8 μg· ml^-1 for Ginsenoside Rb1 （r = 0. 999 9）. The average recovery of Notoginsenoside Rl , Ginsenoside Rg1 and Ginsenoside Rbl was 96. 47% with RSD of 1.52% ,98.75% with RSD of 1.73% and 97.55% with RSD of 1.81% ,respectively. Conclu sion： The method is simple, feasible,precise, sensitive and strongly specific, and can be used for the quality control of Changtai capsules.
出处
《中国药师》
CAS
2011年第10期1447-1449,共3页
China Pharmacist
