摘要
目的研究大肠癌患者K-ras基因的突变情况及其与临床病理特征间的关系。方法应用RT-PCR方法检测148例大肠癌患者组织标本K-ras基因第12和13密码子的突变情况,并与其临床病理特征进行对照。结果 148例大肠癌患者K-ras基因突变率为31.8%(47/148)。K-ras基因第12和13密码子突变在不同性别、肿瘤直径、大体形态、肿瘤部位、远处及淋巴结转移方面差异无统计学意义(P>0.05),在不同年龄与不同临床分期上差异有统计学意义(P<0.05)。结论 RT-PCR可准确检测K-ras基因突变情况,K-ras基因突变与患者年龄、肿瘤临床分期关系密切。
Objective To investigate the mutation of K-ras gene in colorectal cancer and its correlation with clinical and pathological features.Methods A total of 148 colorectal cancer tissue samples were detected the mutation of codon 12 and codon 13 in K-ras gene with reverse transcription-PCR assay and compared with their clinicopathological features.Results The mutation rate of K-ras gene in 148 cases of sporadic colorectal cancer was 31.8%（47/148）.The mutation of codon 12 and 13 of K-ras gene was not significantly different in gender,age,diameter of tumor,gross morphology,tumor location,distant metastasis or lymph node metastasis（P0.05）,but was significantly different in age and clinical stage（P0.05）.Conclusion Reverse transcription-PCR can accurately detect K-ras gene mutation,which is closely correlated with patient＇s age and clinical stage.
出处
《中华实用诊断与治疗杂志》
2011年第9期850-852,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
河南省社会公益项目预研专项课题基金(082103810508)
