摘要
建立离子排斥色谱法测定绿茶中没食子酸的分析方法。采用Metrosep Organic Acids(250mm×7.8mm)分离柱和电导检测器,对分离条件进行优化。结果表明:最佳的色谱条件为以0.006mmol/L H2SO4溶液为淋洗液、50.0mmol/L LiCl溶液为再生液、流速0.5mL/min;在优化的分离条件下,绿茶中常见的F-和8种酸对没食子酸的测定均无干扰,总分析时间为19min,没食子酸的检出限为0.1μg/mL;连续6次进样,峰面积相对标准偏差为1.34%,4种绿茶中没食子酸的回收率为87.1%～96.7%。该方法简单、快速,结果令人满意。
An ion exclusion chromatographic method for the determination of gallic acid in green tea was described.The optimal chromatographic separation was investigated on Metrosep Organic Acids（250 mm×7.8 mm） column with conductivity detection.The optimal separation was achieved using 0.006 mmol/L sulfuric acid as the eluent,50.0 mmol/L lithium chloride as the regenerated liquid at a flow rate of 0.5 mL/min.Fluoride and 8 common acids in green tea had no interference on the determination.The total analysis time was 19 minutes.The detection limit of gallic acid was 0.1 μg/mL.The RSD of peak area for six replicate determinations was 1.34%.The recovery percentages in 4 green teas were 87.1%-96.7 %.So,the method has the advantages of simplicity,rapidity and satisfying results.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第18期253-255,共3页
Food Science
基金
重庆市自然科学基金项目(CSTC2007BB5370)
科技部重大专项(2008ZX07315)
关键词
没食子酸
离子排斥色谱
抑制电导检测
绿茶
gallic acid
ion-exclusion chromatography
suppressed conductivity detection
green tea
