摘要
目的:研究酪蛋白磷酸肽钙磷复合体(casein phosphopeptide-amorphic calcium phosphate,CPP-ACP)对黏性放线菌黏附的影响,进一步探讨CPP-ACP的防龋机制。方法:采用唾液包被的羟磷灰石(saliva-coated hydroxyapatite,S-HA)形成实验性膜的体外黏附实验模型,用不同浓度(0.5%～5.0%(W/V))CPP-ACP处理S-HA,定量观察黏性放线菌在S-HA上的黏附情况。结果:黏性放线菌对经各实验浓度的CPP-ACP处理后的S-HA黏附能力明显下降,黏附量(CPM)随CPP-ACP浓度的升高而降低,且黏附抑制率均达100%。结论:CPP-ACP对黏性放线菌在S-HA上的黏附具有抑制作用,随CPP-ACP浓度的升高其抑制作用增强。
Objective： To investigate the effects of casein phosphopeptide-amorphic calcium phosphate（CPP-ACP） on the adherence of actinomyces viscosus,then evaluate the possibility of CPP-ACP as an effective biological anti-caries agent.Methods： The hydroxyapatite（HA） beads were coated by saliva to form saliva-coated hydroxyapatite（S-HA）,as the adhesion model in vitro.Actinomyces viscosus was chosen as the experimental strain,and radiolabeled with 3H-thymidine.S-HA was treated with different concentrations（0.5%～5.0%（W/V）） of CPP-ACP.The number of 3H-thymidine labeled bacteria attached to S-HA was counted by scintillation counting.Results： CPP-ACP inhibited the adherence of actinomyces viscosus to S-HA effectively.With the concentration of CPP-ACP increasing,the CPM value of experimental groups dropped（P〈0.01）,and all the inhibition rate of adherence reached 100%.Conclusion： CPP-ACP can inhibit the adherence of actinomyces viscosus to S-HA significantly.With the concentration of CPP-ACP increasing,the adhesive inhibition enhances.
出处
《泸州医学院学报》
2011年第5期627-629,共3页
Journal of Luzhou Medical College
基金
泸州市科委科技计划项目资助(01-14/343)
