摘要
目的:观察阿给炒炭前后对止血作用的影响,并初步探讨其止血作用的部分机理。方法:采用家兔外伤出血实验及试管凝血实验,观察阿给炮制前后对家兔出、凝血时间的影响。采用凝固法、比浊法、试管法测定家兔给药前后凝血酶原时间(PT)、活化部分凝血酶原时间(APTT)、凝血酶时间(TT)、纤维蛋白原含量(FIB)、血浆复钙时间(PRT)、优球蛋白溶解时间(ELT)、血小板最大聚集率(MPAR),分析阿给炮制前后对家兔凝血、纤溶系统及血小板功能的影响。结果:阿给生药中、高剂量组和炭药各剂量组均能明显缩短家兔凝血时间(P<0.01或P<0.05);生药和炭药各剂量组均能明显缩短家兔出血时间(P<0.01或P<0.05)。阿给生药及炭药各剂量组对PT均无显著影响(P>0.05);生药高剂量组和炭药各剂量组能明显缩短APTT(P<0.01或P<0.05)、延长ELT(P<0.01或P<0.05);生药高剂量组和炭药高剂量组均能明显缩短TT(P<0.01或P<0.05);炭药中、高剂量组能明显增加FIB(P<0.01或P<0.05);炭药高剂量组能显著缩短PRT(P<0.05)、增加MPAR(P<0.01)。结论:阿给生药能通过影响内源性凝血途径、纤溶系统来发挥其止血促凝作用,炮制后作用效果有增强的趋势,且炭药能显著增大FIB和MPAR,更有利于促进血液的凝固。
Objective：To observe the effects of raw and charred Agi on hemostasis and its mechanism.Methods：The rabbit bleeding time was measured by traumatic hemorrhage test,and the clotting time was measured by tube test.The rabbit prothrombin time（PT）,activated partial thormboplastia time（APTT）,thrombin time（TT）,fibrinogen（FIB）,plasma recalcification time（PRT）,euglobulin lysis time（ELT）,max platelet aggregation rate（MPAR）were measured by solidification method,turbidimetry and tube test to analyze the effects of raw and charred Agi on rabbit coagulation-fibrinolysis system and platelet function.Results：The medium doses and high doses of raw Agi groups,all of the groups of charred Agi decreased rabbit BT obviously（P〈0.01 or P〈0.05）;all of the groups of raw and charred Agi declined rabbits CT（P〈0.01 or P〈0.05）.All of the doses groups of raw and charred Agi had no apparent influences on PT（P〉0.05）;the high dose of raw Agi group and all of the groups of charred Agi decreased APTT apparently（P〈0.01 or P〈0.05）and prolonged ELT（P〈0.01 or P〈0.05）;the high doses groups of raw and charred Agi decreased TT apparently（P〈0.01 or P〈0.05）;the medium and high doses groups of charred Agi increased FIB obviously（P〈0.01 or P〈0.05）;the high doses group of charred Agi showed the decreased PRT significantly（P〈0.05）and increased MPAR obviously（P〈0.01）.Conclusions：Raw Agi can play its role in hemostasis and coagulation by affecting the intrinsic pathway of coagulation and fibrinolytic system.These effects are inhanced after processing drugs;moreover,the charred Agi could increase FIB and MPAR with promoting more in blood coagulation.
出处
《中药材》
CAS
CSCD
北大核心
2011年第6期872-876,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金资助项目(81072894)
关键词
蒙药阿给
炒炭
止血作用
作用机制
Mongolian drug Agi
Processing
hemostatic effects
mechanism of action
