摘要
FBPase是调控Clavin循环的关键酶之一,加速Clavin循环有利于提高植物的光合效率。在油茶cDNA文库和EST文库构建的基础上,以油茶"湘林1号"的近成熟种子为材料,采用5’RACE和3’RACE技术克隆油茶FBPase基因的全长cDNA序列;该基因全长cDNA序列为1 356 bp,其中开放读码框为1 020 bp,多序列比对发现其与毛果杨的亲缘关系最近,相似性为84%;该基因共编码339个氨基酸,其蛋白质属稳定蛋白质,等电点为5.54,不含二硫键,为胞质FBPase,将该基因命名为co-fbp。油茶FBPase基因的克隆对于进一步研究油茶光合作用、提高油茶光能利用率、提高油茶产量具有重要的意义。
FBPase is one of the key enzymes controlling Calvin Cycle,Which helps to improve Clavin circulation to accelerate photosynthetic efficiency of plants.The full length cDNA of FBPase gene was separated from the EST library and the cDNA library of Camellia oleifera by the method of 5＇RACE and 3＇RACE.The results showed that this gene is 1 356 bp in length,and it has an open reading frame of 1 020 bp,and the alignment of nucleotide acids sequence reveals that the comparability of Camellia oleifera and populus trichocarpa is 84%.This gene encodes 339 aminoacids,the protein is a stable protein whose Isoelectric point is 5.54.Camellia oleifera FBPase encoded protein does not contain disulfide,thus the gene is c-FBPase named co-fbp.This study is significant help to the research on the photosynthesis of Camellia oleifera,increaseing energy efficiency and production.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2011年第3期514-520,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(31070603)
