摘要
以来自“掖单 4号”的玉米果糖 6 磷酸 ,2 激酶 果糖 2 ,6 二磷酸酶 (F2KP)基因cDNA片段 (AF0 0 75 82 )为基础 ,运用RT PCR和RACE技术 ,从“紫玉糯 1号”中获得了 1个 2 4 6 9bp的玉米F2KP基因cDNA克隆 ,命名为mF2KP ,GenBank登录号为AF33414 3。该cDNA包含 1个 2 2 2 6bp的开放阅读框 ,编码 74 1个氨基酸。序列分析表明 ,两个玉米品种的F2KP基因存在一定差异 :mF2KP基因的 3′端非编码区比AF0 0 75 82序列短 38bp ;在mF2KP的15 92、15 93和 16 0 5位置上 ,分别比AF0 0 75 82序列多出了 1个碱基 ,导致阅读框在一个小范围内发生了移位。North ern杂交表明 :不同玉米组织中mF2KP的表达差异明显。在茎中mF2KP的表达水平比叶片、苞叶以及雄花序中的表达水平低 ,但比未成熟种子中的表达水平高。在未成熟种子中 。
A 2469 bp cDNA encoding entire fructose 6 phosphate,2 kinase/fructose 2,6 bisphosphatase (F2KP) was cloned from maize ( Zea mays L.cv.Ziyunuo 1) by the methods of RT PCR and rapid amplification of cDNA ends(RACE),on the basis of AF007582,which was isolated from 'Yedan 4'.The cDNA was designated as mF2KP and the GenBank accession number is AF334143,which contains a 2226 bp open reading frame (ORF),encoding a 741 residue polypeptide.There are some differences between the F2KP genes of two maize varieties.The length of 3' non coding region of mF2KP is 38 bp shorter than that of AF007582.On the 1592th,1593th and 1605th positions of mF2KP ,there is an additional nucleotide respectively compared with AF007582,which cause a shifted reading frame in a small region.Northern blot showed that the expression of mF2KP were significantly different among maize tissues.The Transcription of mF2KP in stem was lower than those in leaves,kernel leaves and male inflorescence,but much higher than that in immature seeds.
