摘要
采用生物信息学方法,从随机测序而构建的油茶优良无性系湘林1号种子EST文库中初步鉴定了与茶油脂肪酸形成相关的大部分关键酶基因,并对其中几个直接控制不饱和脂肪酸的形成与转化的基因进行了相应的序列分析．
In order to determine the molecular mechanism of forming high quality tea-oil, the EST library technology was used to find out the main expressed genes controlling the fatty acid biosynthesis in camellia oleifera seeds. A high quality full-length cDNA library was constructed successfully using the nearly matured seeds of elite cultivar Xianglin-1 planted widely in China, then more than two thousand clones were randomly sequenced from the 5＇ orientation, which was used to construct a high representational EST library. After the bioinformatic analysis of this EST library, 15 main genes controlling the biosynthesis of unsaturated fatty acids in camellia oleifera seeds were obtained.
出处
《经济林研究》
2007年第2期5-8,共4页
Non-wood Forest Research
基金
Supported by national natural science found of China(30371184)
natural science found of Hunan(05JJ40125)
young teacher's scientific research found of CSUFT(05007A).
关键词
油茶
脂肪酸
EST文库
表达基因
生物信息学
Camellia oleifera
fatty acids
EST library
expressed genes
bioinformatics
