摘要
为了进一步明确从土壤中筛选得到的拮抗细菌OR-1、OR-2、OR-3、ON-6的分类地位和生防效果,采用形态学观察、理化特性结合分子生物学方法,鉴定这4株细菌皆为枯草芽胞杆菌Bacillussubtilis。拮抗菌对荔枝霜疫霉菌、炭疽菌菌丝生长均具有明显抑制作用,ON-6菌株的发酵液对菌丝生长的抑制率最高,分别为92.34%和70.36%,其次是OR-1菌株。拮抗菌处理组褐变指数均小于对照组以及杀菌剂处理组,且差异达显著水平(P<0.05),OR-1菌株防褐变效果最好。常温下,拮抗菌发酵液对离体鲜果病害防治效果优于对照组和杀菌剂处理组,其中ON-6菌株的防治效果最佳;4℃低温储藏处理40天后,拮抗菌发酵液处理组的防治效果高于对照和杀菌剂处理组,且差异显著(P<0.05),以ON-6和ON-3菌株的防治效果最佳。表明拮抗菌发酵液对荔枝鲜果上的霜疫霉菌和炭疽病菌有一定的抑制作用。
Four antagonistic bacteria strains,OR-1,OR-2,OR-3 and ON-6,which were isolated and screened from soil under litchi trees and identified as Bacillus subtilis morphologically and molecularly,were used to evaluate the control effect on downy mildew and anthracnose diseases of harvested litchi fruits.The results showed that all 4 strains had obvious inhibiting action on mycelial growth of Peronophythora litchii and Colletrichum gloeosporiodes,among them,ON-6 strain had the highest inhibitory rate to mycelial growth of two litchi pathogenic fungi,the ratio were 92.34% and 70.36% respectively;the next best was ON-1,its ratio were 82.93% and 54.61% severally.The browning index of the four strains treatment groups was lower than that of the control group and fungicide groups significantly（P0.05）,in which OR-1strain had the best impact.Metabolites of four B.subtilis strains had a better effect in pre-venting P.litchii and C.gloeosporiodes on the surface of fresh fruits than those of CK group and fungicide groups at room temperature for 3-4 d or at 4℃ for 40d significantly（P0.05）,which ON-6 strain showed best at room temperature,likewise,ON-6 and ON-3 strains showed best at 4℃,indicated that the four B.subtilis strains could inhibit litchi pathogenic fungi on the surface of fruits determinately,and extend the period of storage and delay the fresh fruits to brown stain at room temperature or at 4℃.
出处
《植物保护学报》
CAS
CSCD
北大核心
2011年第3期247-252,共6页
Journal of Plant Protection
基金
国家自然科学基金(30860010)
广西科学基金(桂科回0832006)
广西科研院所基本科研业务费资助项目(08YJ16WL01)
关键词
枯草芽胞杆菌
荔枝
霜疫霉菌
炭疽病菌
拮抗作用
Bacillus subtilis
Litchi chinensis
Peronophythora litchi
Colletotrichum gloeosporioides
antagonistic
