摘要
枯草芽孢杆菌JA分泌物中具有抑菌作用的粗提液通过DEAE SepharoseFF、SOURCE 1 5PHE、Sephacry1S 2 0 0HR和反相HPLC多步柱层析分离纯化后 ,获得 3种对水稻纹枯和小麦赤霉病菌均有抑菌作用的抗菌肽AFP1、AFP2和AFP3。MALDI TOF质谱法测得其分子量分别为 1 4 6 2 6 4 5D、1 4 76 390D和 1 4 90 5 30D。氨基酸组成分析结果表明 ,AFP1和AFP3由苏氨酸、异亮氨酸、酪氨酸、脯氨酸等多种氨基酸组成。目标产物热稳定性好、对蛋白酶有一定的耐受性 。
Three types of antimicrobial peptides, named AFP1、AFP2and AFP3,which all had strong inhibitory activity against Rhizoctonia solani and Fusarium graminearum were purified from the crude extract of Bacillus subtilis JA. Their molecular weights were1462 645D, 1476 390D and 1490 530D respectively, determined by MALDI TOF mass spectrometry. The amino acid compositions of the two antimicrobial peptides were analyzed and the results showed that the purified materials were composed of Thr, Ile, Pro, Val, Glx (Glu or Gln)and so on. The peptides were found to be thermostable and partial resisted proteinases. It appeared that the peptides may be cyclic lipopetides.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第4期511-514,共4页
Acta Microbiologica Sinica
基金
国家攻关项目 ( 2 0 0 1BA3 0 2B 4)
安徽省自然科学基金项目 ( 0 10 414 0 1)~~
关键词
枯草芽孢杆菌
抗菌肽
分离
纯化
氨基酸
Bacillus subilis JA, Purification, Antibacterial peptide
