摘要
目的探讨海带多糖(LJP)对放疗后颌下腺细胞凋亡的影响。方法将Wistar大鼠随机分为4组:正常对照组、放疗对照组,放疗LJP A组(LJP 30 mg/kg)及放疗LJP B组(LJP 300 mg/kg)。放疗对照组、放疗LJP A组及放疗LJP B组的大鼠颌下腺给予一次性γ射线照射,每只总剂量15 Gy。采用免疫组化法及原位切口末端标记(TUNEL)法分别检测大鼠颌下腺细胞的Bcl-2,Bax表达及凋亡情况。结果与放疗对照组比较,放疗LJP A组及放疗LJP B组大鼠颌下腺细胞的Bcl-2表达水平明显增强(P<0.05),Bax水平无明显改变(P>0.05);与正常对照组比较,放疗对照组的凋亡细胞明显增加(P<0.05),而与放疗对照组比较,放疗LJP A组及放疗LJP B组的凋亡细胞明显减少(P<0.05)。结论 LJP可调节细胞Bcl-2及Bax的表达水平,抑制细胞凋亡,从而保护颌下腺免受放疗损伤。
Objective To explore effects of laminaria japonica polysaccharides（LJP） on apoptosis of submandibular gland cells induced by radiation in rats.Methods Wistar rats were randomly divided into 4 groups：normal group,radiation control group,radiation LJP A group（LJP 30 mg/kg） and radiation LJP B group（LJP 300 mg/kg）.Each rat in radiation control group,radiation LJP A group and radiation LJP B group was received a single and total dose of 15Gy γ-irradiation on the area of rat submandibular gland.Immunohistochemistry and TdT-mediated dUTP nick end labeling（TUNEL） methods were employed to detect expression of Bcl-2,Bax and apoptosis of submandibular gland cell in rats.Results Compared with radiation control group,levels of Bcl-2 expression of submandibular cells of rats in radiation LJP A and radiation LJP B groups were increased（P0.05） while that of Bax had no significantly change（P0.05）;the number of apoptotic cells in radiation control group were increased markedly compared to normal group（P0.05）,and it were decreased obviously in radiation LJP A group and radiation LJP B group compared to radiation control group（P0.05）.Conclusion LJP has protective effects on submandibular gland against radiation injury via regulation levels of Bcl-2 and Bax expression and inhibition cellular apoptosis.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第15期1466-1467,1470,I0001,I0002,共5页
Chongqing medicine
基金
广西科学基金资助项目(桂科自0728241)
关键词
颌下腺
细胞凋亡
海带多糖
放射损伤
sabmandibular gland
apoptosis
laminaria japonica polysaccharides
irradiation damage
