摘要
目的：建立大鼠实验性牙髓炎模型；原位观察大鼠正常牙髓和炎症牙髓中Ｂｃｌ－２和Ｂａｘ的表达情况，探讨两者在牙髓炎病理变化中的作用。方法：利用ＳＤ大鼠的下颌第一磨牙采用内毒素化学诱导法建立牙髓炎模型，用免疫组织化学染色的方法研究Ｂｃｌ－２和Ｂａｘ的表达及其意义。结果：正常牙髓中Ｂｃｌ－２在成牙本质细胞层表达阳性，牙髓中央区域弱阳性，Ｂａｘ在成牙本质细胞层表达强阳性，牙髓中央区域阳性。在牙髓中央的表达弱于牙髓外周，Ｂｃｌ－２的表达在相应部位弱于Ｂａｘ。在炎症牙髓中炎细胞浸润区Ｂｃｌ－２表达强阳性，纤维包饶区和充血反应区表达分别为阳性和弱阳性，Ｂａｘ在炎症牙髓中着色较均匀。结论：成牙本质细胞凋亡与Ｂｃｌ－２和Ｂａｘ的表达量有关，正常牙髓中存在细胞凋亡，这与该部位Ｂａｘ表达量较高有关；Ｂｃｌ－２和Ｂａｘ是牙髓炎时炎细胞凋亡的重要调控因素之一。
Aims: To establish a model of rat endotoxic pulpitis. To observe the expression of Bcl-2 and Bax in rat normal and inflammatory dental pulp in situ, and to explore the impact of Bcl-2 and Bax on pulpitis. Methods: Sixteen rat molars by application of LPS were removed from the jaws after 24h、72h、120h and 168h respectively. The expression of Bcl-2 and Bax was assessed with immunohistochemical technique. Results: Many Bcl-2 and Bax positive cells were observed in odontoblasts of rat normal pulp, and few of them were in the middle of pulp. The positive rate and staining intensity of Bax were significantly higher than the Bcl-2 in the same area. Conclusions:The apoptosis of odontoblasts was related to the expression of Bcl-2 and Bax. Apoptosis in dental pulp was accompanied with a higher expression of Bax. Bcl-2 was one of the important modulated factors of apoptosis in pulpitis, and Bax was more stable.
出处
《牙体牙髓牙周病学杂志》
CAS
1999年第1期55-57,共3页
Chinese Journal of Conservative Dentistry
