摘要
研究雌二醇(E2)与双酚A(BPA)联合作用于乳腺癌MCF-7细胞后,对细胞内ERK信号转导通路的影响.运用Western blot法进行实验.测定增殖相关蛋白Ki67,观察到E2与BPA单独及联合后都能够诱导细胞增殖,发挥雌激素活性.通过对ERK蛋白和活化形式p-ERK蛋白的测定,E2及与BPA联合后的雌激素活性发挥通过ERK信号通路,激活了转录因子c-Myc蛋白.E2与BPA联合后的雌激素活性较E2单独作用时减弱.
In order to study the effect of E2 and BPA on ERK signaling pathway,MCF-7 breast cancer cells were used to test.Western blot methods were used.Ki67,ERK,and c-Myc proteins were determinated.The results showed that E2,BPA and the mixture could induce the proliferation of MCF-7cells,E2 and the mixture play the estrogenic activities by ERK signaling pathway,and activate the transcription factor c-Myc protein.The estrogenic activities of E2 mixed with BPA weaken than E2 single.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2011年第2期162-164,173,共4页
Journal of Harbin University of Commerce：Natural Sciences Edition
