摘要
为初步探讨雌激素对骨的作用机制 ,以体外培养的小鼠胚胎长骨作为模型 ,通过免疫组织化学方法检测雌酚酮对骺板静止区、增殖区和肥大区 c- myc蛋白表达的影响。结果显示 ,培养介质中雌酚酮浓度为10 - 9mol/ L 时 ,增殖区 c- m yc蛋白阳性细胞面积与对照组相比显著增加 ;浓度为 10 - 8mol/ L 时 ,除增殖区外 ,静止区阳性细胞面积也显著增加 ;当浓度增至 10 - 7mol/ L 时 ,3个区阳性细胞面积均显著增加。提示雌激素可通过促进长骨 c-
In order to study the mechanism by which estrogen effects on bone, the effect of estrone on expression of c myc protein in the epiphyseal plate of fetal mouse long bone was detected by immunohistochemistry procedure in vitro. The result showed that the concentrations of estrone areas of positively staining cells increased significantly in proliferative zone at 10 -9 mol/L, in proliferative zone and resting zone at 10 -8 mol/L, and in resting zone, proliferative zone and hypertrophic zone at 10 -7 mol/L respectively. It was considered that estrogen might enforce endochondral bone formation by enhancing expression of c myc in long bone.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第6期402-404,共3页
Journal of Hygiene Research
