摘要
目的研究AB-8大孔吸附树脂富集纯化黄药子总皂苷的工艺条件。方法建立高氯酸比色法测定黄药子总皂苷含量,以黄药子总皂苷含量为指标,考察AB-8大孔吸附树脂富集纯化黄药子总皂苷的吸附性能和洗脱条件。结果黄药子样品液与树脂柱最佳体积比为15∶1,30%～70%乙醇洗脱时,纯度为74.9%。结论 AB-8型吸附树脂能够较好地富集纯化黄药子总皂苷,优选出的工艺条件科学合理、简便高效,便于推广。
Objective To study the AB-8 macroporous resin enrichment and purification of total saponins from Airpotato yam.Methods Perchloric acid was used to determine the total saponins content of Airpotato yam.Total saponin content in Airpotato yam was used as an index to determine adsorption and elution conditions by AB-8 macroporous resin enrichment and purification of total saponins of Airpotato yam.Results The optimum volume ratio between Airpotato yam sample solution and resin column was 15∶1.When eluted by 30%-70% ethanol,the purity of Airpotato yam total saponins extract was 74.9%.Conclusion Total saponins of Airpotato yam is better enriched and purified by AB-8 macroporous resin.The condition is scientific,rational,simple and efficient.
出处
《中南药学》
CAS
2011年第4期255-257,共3页
Central South Pharmacy
基金
江苏省中药药效与安全性评价重点实验室开放研究课题(T09002)
关键词
大孔吸附树脂
黄药子总皂苷
富集纯化
macroporous resin； total saponins of Airpotato yam； enrichment and purification
