摘要
目的建立高效液相色谱法同时测定大孔吸附树脂富集纯化后的黄芩提取物中黄芩苷、黄芩素和汉黄芩素含量的方法。方法以AB．8型大孔吸附树脂对黄芩水提取物中总黄酮进行富集纯化，采用高效液相色谱法同时测定其中黄芩苷、黄芩素和汉黄芩素的含量。色谱柱：AgilentTC—C18（4．6mmx250mm，5μm）；流动相：乙腈-0．1％磷酸水溶液，梯度洗脱；检测波长：280nm；流速1．0mL·min-1；柱温：30℃。结果黄芩苷、黄芩素和汉黄芩素分别在0．0224～0．4480gg（r=0．9999），0．0210-0．4200μg（r=0．9999），0．0103-0．2050μg（r=0．9999）内线性关系良好；平均回收率分别为97．3％（RSD1．71％），98．4％（RSD2．05％），98．1％（RSD1．65％）。黄芩水提取物经大孔吸附树脂纯化后，黄芩苷、黄芩素和汉黄芩素的平均含量分别为27．52％、6．87％和2．13％，未经纯化的黄芩水提取物中三者的平均含量分别为9．16％、1．66％和0．54％。
OBJECTIVE To establish a method for determination of the content of baicalin, baicalein and wogonin in Scutellaria baicalensis purified by macroporous absorption resin by HPLC. METHODS The macroporous absorption resin AB-8 was used to separate and purify the total flavonoids in the water extract from Scutellaria baicalensis. The HPLC method was used to determine the content of baicalin, baicalein and wogonin. The analytical column was Agilent TC-C18（4.6 mm ×250 mm, 5 μm）. The mobile phase was a mixture liquid of acetonitrile and 0.1% phosphoric acid in water by gradient elution. The UV detector wavelength was 280 nm, the flow rate was 1.0 mL.min-1, and the column temperature was 30℃. RESULTS The linear ranges of baicalin, baicalein and wogonin were 0.022 4-0.448 0 μg（r=0.999 9）, 0.021 0-0.420 0μg（r=0.999 9）, 0.010 3-0.205 0 μg（r=0.999 9）, respectively. The average recoveries of the method for baicalin, baicalein and wogonin were 97.3% with RSD 1.71%, 98.4% with RSD 2.05% and 98.1% with RSD 1.65%, respectively. The average contents of baicalin, baicalein and wogonin in the water extract from Scutellaria baicalensis with macroporous absorption resin were 27.52%, 6.87% and 2.13%, and the average contents were 9.16%, 1.66% and 0.54% without purification. CONCLUSION The method is simple, accurate, sensitive and stable. KEY WORDS： HPLC; macroporous absorption resin; baicalin; baicalein; wogonin
出处
《中国现代应用药学》
CAS
CSCD
2013年第10期1107-1110,共4页
Chinese Journal of Modern Applied Pharmacy
基金
辽宁省教育厅重点实验室项目(LS2010105)
