摘要
【目的】探讨铁皮石斛种子萌发、芽增殖和生根的适宜培养基,为铁皮石斛组织快繁技术提供依据。【方法】以铁皮石斛种子为外植体进行培养,研究6-BA(0-3.0mg/L)、NAA(0.1-0.3mg/L)、香蕉泥(15%～25%)和马铃薯泥(15%～25%)对铁皮石斛种子萌发、芽增殖和生根壮苗的影响。【结果】适宜种子萌发的较好培养基为MS+20%马铃薯泥,芽增殖最适培养基为MS+2.0mg/L6-BA,增殖率达282.7%;最适生根培养基为MS+15%香蕉泥,生根率达100%。【结论】经马铃薯泥、香蕉泥培养的铁皮石斛组培苗,生长健壮,能为生产提供大量的种苗,因而此类培养基可用于大规模生产铁皮石斛组培苗。
[Objective]The experiments were conducted to find out the suitable culture medium for the seeds germination,bud multiplication,rooting and growth of plantlet in Dendrobium candidum Wall.ex Lindl,and to develop a technique for its rapid propagation. [Method]Seeds of Dendrobium candidum were used as explants in tissue culture,and the effects of different media with different concentrations of 6-BA （0-3.0 mg/L） and NAA（0.1-0.3 mg/L）,supplemented with mashed banana （15-25%） or mashed potato（15-25%）,on the seed germination,bud multiplication,rooting and growth of plantlets were investigated. [Result]The most suitable culture medium for seed germination and bud multiplication was MS＋20% mashed potato and MS＋6-BA （2.0 mg/L）,respectively. However,the best suitable medium for rooting was found MS＋15% mashed banana which gave 100% rooting rate. [Conclusion]The strong rooting plantlet can be obtained in medium supplemented with mashed potato and mashed banana. This medium may be recommended for the large scale production of Dendrobium candidum plantlets.
出处
《南方农业学报》
CAS
CSCD
2011年第4期349-352,共4页
Journal of Southern Agriculture
关键词
铁皮石斛
组织培养
种子萌发
芽增殖
生根
Dendrobium candidum Wall.ex Lindl
tissue culture
seed germination
bud multiplication
rooting
