摘要
对珍稀濒危植物铁皮石斛的种子、原球茎和类原球茎体进行了液氮超低温保存研究。把４个月龄的种子脱水至含水量为１２％～１９％，能获得较高的冻后存活率（９５％）和较快的恢复生长速率。种子在０．５ｍｏｌ／Ｌ脱落酸培养基上萌发３周所形成的原球茎，用玻璃化保护剂ＰＶＳ２处理１５ｍｉｎ，其冻后存活率可达８８％。将生长旺盛的暗培养类原球茎体的含水量在６～７ｄ内干燥至３０％±２％，其冻后存活率可达４８％～８０％。
Seeds,protocorms and protocormlikebodies of the rare and endangered plant Dendrobium candidum were successfully cryopreserved.High survival rates(about 95%)and rapid recovery growth could be achieved by drying the fourmonthold seeds to water contents ranging from 12 to 19%.Survival rates of 88% were obtained when protocorms,cultured for 3 weeks on media containing 0.5 mg/L abscisic acid,were incubated for 15 min in the plant vitrification solution PVS 2 and then exposed to liquid nitrogen.Darkcultured protocormlikebodies in vigorous growth were desiccated to a water content of 30%2% through 6-7 days.After cryopreservation,their survival rates were 48% to 80%.
出处
《园艺学报》
CAS
CSCD
北大核心
1999年第1期59-61,共3页
Acta Horticulturae Sinica
基金
国家自然科学基金
浙江省自然科学基金
