摘要
为了发挥微生物和植物在重金属矿尾区铜污染土壤的共同修复作用,本研究从陕西省凤县矿尾区天蓝苜蓿根瘤中筛选分离到一株抗重金属铜的菌株,定名为CCNWSX0020。该菌株在YMA琼脂平板上抗铜浓度为3.0mmol/L,TY液体培养基中抗铜浓度为1.4 mmol/L,因此,它是研究根瘤菌抗铜机制的良好菌株。该菌株经生理生化分析、G+C mol%测定、DNA同源性分析及16S rDNA序列相似性比较,鉴定为苜蓿中华根瘤菌(Sinorhizobium melilo-ti),它与苜蓿中华根瘤菌LMG6133的菌株(GenBank登录号为X67222)相似性为99.7%。由于根瘤菌具有独特的生物学地位,即能够与豆科植物形成共生体,这为进一步构建耐重金属的生物体系提供了良好的遗传基础,具有潜在的生态效益和社会效益。
In recent years,with the development of mining,smelting and processing of copper industry,soil copper pollution is becoming increasingly serious.Thus it is imperative to restore the soil copper contamination.In this study,in order to develop collaborative restoration activity of microorganisms and plants in the copper-contaminated soils of heavy metals tailing area,an excellent Rhizobium strain resistance to copper was screened from mining tailings Fengxian county,Shaanxi province.The strain,named CCNWSX0020,resisted to 3.0 mmol/L Cu2＋ on YMA agar plate and to 1.4 mmol/L Cu2＋ in TY liquid medium,so it is an important strain using to study the resistant copper mechanisms of microbe.It was preliminarily identified as a strain of Sinorhizobium meliloti,based on its physiological and biochemical characteristics analysis,including G＋C mol% determination,DNA homology hybridization and 16S rDNA sequence similarity comparison.The similarity between CCNWSX0020 and S.meliloti LMG6133（GenBank accession number X67222）was 99.7%.Because of Rhizobia with the unique biology status that is being able to form symbiont with legume,which can provide a good genetic base for the further construction of a biology system being tolerant to heavy metals,and as a result,having potential ecological and social benefits.
出处
《华北农学报》
CSCD
北大核心
2011年第2期7-12,共6页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金(310704443097000330900215)
