摘要
在陕甘宁地区根瘤菌资源调查的基础上，选取其中与黄芪、岩黄蓍、木兰、鸡眼草及胡枝子共生的４０株快生根瘤菌和２５株参比菌株，对１３３项表型性状进行聚类分析，在８２％的相似性水平上构成５个不同于已知种的新亚群．对第１、２、３、４亚群ｘ（Ｇ＋Ｃ）／％测定及ＤＮＡ同源性分析结果表明，４个亚群ｘ（Ｇ＋Ｃ）／％含量均处于Ｒｈｉｚｏｂｉｕｍ属范围；各亚群内菌株ＤＮＡ同源性均在７５％以上，每个亚群中心菌株与１３个已知种模式菌株的ＤＮＡ同源性均小于６３％．从而证明，第１、２、３。
Based on the investigations of rhizobial resources of Shaanxi, Gansu and Ningxia, 40 fast growing rhizobia strains isolated from Astragalus,Hedysarum, Indigofera,Kummerowia and Lespedeza bicolor, and 25 reference strains were numerically classified and analyzed with 133 phenotypic features.The new isolated strains were divided into five new subgroups at 82% similarity level.Subgroups 1,2,3 and 4 were further studied by x (G+C)/% and DNA homology analysis.The x (G+C)/% of subgroups 1,2,3,4 belong to Rhizobium , DNA similarities of the subgroups were ≥75%,and DNA similarities between the central strains of new subgroups and the type strains of 13 known species were less than 63%.Thus, subgroups 1,2,3 and 4 were four new individual species groups of Rhizobium.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第1期73-78,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金
