摘要
将Lmbr1基因作为鸡多趾研究的候选基因,寻找与多趾有关的SNPs并进行相关性分析研究。通过PCR-SSCP及直接测序,在北京油鸡(四趾/五趾)Lmbr1基因第16外显子检测到2个位点存在同义突变(c.1326A>G和c.1360C>A),5种基因型(AABB、aaBB、AABb、AaBb、AaBB)。对c.1326A>G、c.1360C>A两位点在不同趾型的基因频率及基因型进行统计分析,结果发现,在c.1326A>G位点,双五趾和混合趾中a为优势等位基因(0.83、0.81),双四趾中A为优势等位基因(0.53),双五趾和混合趾中aa为优势基因型(0.650、0.625),双四趾中Aa为优势基因型(0.420),AA基因型仅出现在双四趾中,在双五趾和混合趾中没有发现。在c.1360C>A位点,双五趾、混合趾和双四趾中B均为优势等位基因(0.85、0.88、0.76);双五趾、混合趾和双四趾中BB均为优势基因型(0.692、0.750、0.516),在试验鸡群中没有发现bb基因型。在北京油鸡群体中,c.1326A>G等位基因处于Hardy-Weinberg非平衡状态(P<0.01),c.1360C>A等位基因处于Hardy-Weinberg平衡状态(P>0.05)。研究结果表明,c.1326A>G位点与鸡趾型密切相关,有望用于标记辅助选择。
Considering Lmbr1 is a key candidate gene of chicken polydactyly,the study on search SNPs which was influence the polydactyl.By the methods of PCR-SSCP and direct sequencing,two synonymous mutation（c.1326AG,c.1360CA） and five genotypes（AABB,aaBB,AABb,AaBb,AaBB） in exon 16 of Lmbr1 gene were detected from Beijing-fatty chicken（4-teod and 5-teod）.The statistical analysis showed that the a allele gene frequencies was advantage on c.1326AC locus of Lmbr1 between double 5-teod and mingle-teod chicken group（0.83,0.81）,A allele gene frequencies was advantage in double 4-teod chicken（0.53）.aa genoptype was preponderance in 5-teod and mingle-teod chicken group（0.650,0.625）,Aa genotype was dominant position in double 4-teod chicken（0.42）,AA was only found on 4-teod chicken group,and was not found in 5-teod and mingle-teod chicken group.B allele gene frequencies was advantage on c.1326AC locus in double 5-teod,mingle-teod and double 4-toed chicken group（0.85,0.88,0.76）,BB genoptype was preponderance in double 5-teod,mingle-teod and double 4-toed chicken group（0.692,0.750,0.516）,and was not found bb genoptype.In the chicken group,by Hardy-Weinberg equilibrium tested,c.1326AC locus was in non-equilibrium,c.1360CA locus was in equilibrium.The research showed that c.1326AG was close associate with polydactyly,the site may utilize to MAS.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第5期114-117,共4页
China Animal Husbandry & Veterinary Medicine
基金
北京市科技合同项目"北京油鸡育种及其关键技术研究"
