摘要
根据人Lmbr1/C7orf2基因的一种缺失外显子4的可变剪接与Acheiropodia(ACHP)疾病的关联,本研究拟根据可变剪接在物种间的保守性,进行鸡Lmbr1这种相似可变剪接的鉴定和表达分析。设计跨越Lmbr1外显子4的引物可同时检测这2种转录产物的表达,本研究成功地从鸡的心脏组织获得了缺失外显子4的Lmbr1异常剪接(Lmbr1-β)。在白来航初生雏鸡及5～6胚龄胚的组织表达分析显示,2种转录产物均可在所检测的组织中表达,与包含外显子4的转录产物(Lmbr1-α)相比,可变剪接Lmbr1-β为一种次要表达产物,表达量较弱。对多趾的丝羽乌骨鸡和四趾的白来航鸡胚组织间2种转录产物表达进行进一步分析,在2个品种3～11胚龄(E3～E11)的胚胎发育期间均可同时检测到Lmbr1-α和Lmbr1-β的表达,Lmbr1-α为主要表达产物,Lmbr1-β为次要表达产物,Lmbr1-β表达量微弱。在2个品种的胚胎发育期间未见2种转录产物明显的表达差异。结果显示,包含外显子4的Lmbr1-α为鸡Lmbr1基因的主要转录产物,广泛而稳定地在雏鸡和发育鸡胚各组织中表达,丝羽乌骨鸡多趾表型的发育与常见转录产物Lmbr1-α和异常可变剪接Lmbr1-β的表达无直接的相关。
Based on the report that the abnormal splice form deleted exon 4 of Lmbr1/C7orf2 associated with human Acheiropodia and the conservation of the alternative splices among species to some degree,the identification and expression analysis of chicken Lmbr1 similar transcript variant were conducted.Primers spanning chicken Lmbr1 exon 4 were designed to clone the alternative splice deleted exon 4（Lmbr1-β）of chicken Lmbr1-α.The primers spanning exon 4 could detect the expression of two different transcripts of chicken Lmbr1 at the same time.The tissue expression analysis in one day-old chicks and 5-6 day-old embryos of White Leghorn showed that two transcripts could be detected in all tissues,and comparing with chicken Lmbr1-α,Lmbr1-β was a minor transcript and its expression level was weak.Comparison between the Lmbr1 expression of E3-E11 in polydactyly Silkies and four tetradactyly White Leghorn showed that both Lmbr1-α and Lmbr1-β expressed in all detected chicken embryo tissues,Lmbr1-α was the main transcript product and Lmbr1-β was a minor transcript product in very weak expression.No significant tissue expression difference was identified between the two breeds.It showed that Lmbr1-α containing exon 4 was the main transcript of Lmbr1,which expressed in all detected tissues of the chicken and developing embryos,the development of polydactyly in Silky was not related with the expression of chicken Lmbr1-α and Lmbr1-β.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第5期518-523,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30771533)
国家"十一五"支撑计划(2008BADB2B01)
郑州市创新型科技人才队伍建设工程科技创新团队(096SYJH16092)
关键词
鸡
Lmbr1
异常可变剪接
克隆
表达
chicken
Lmbr1
abnormal alternative splicing
cloning
expression
