摘要
该文报道了一种基于金银核壳纳米粒子（Au@Ag CS NPs）检测乳品中三聚氰胺的简单、快速电化学方法。通过层层自组装技术将二硫苏糖醇（DTT）、金银核壳纳米粒子（Au@Ag CS NPs）、L-半胱氨酸（L-Cys）键合在金表面,形成Au/DTT/Au@Ag CS NPs/L-Cys修饰传感膜。通过电化学阻抗和循环伏安行为探讨了该修饰电极检测三聚氰胺的作用机理,发现其阻抗值与三聚氰胺浓度对数在1.0×10^-7-1.0×10^-2mol/L范围内呈良好线性关系,线性拟合方程为y=1049.7＋56.75x,线性相关系数为0.9976,检测限为6.553×10^-8mol/L。该修饰电极选择性和重现性好,用于牛奶样品中三聚氰胺的检测,回收率为98.2%-106.0%,有实际应用价值。
A new, simple and rapid electrochemical method was developed for detection of melamine in milk samples based on Au@Ag core-shell nanoparticles（Au@Ag CS NPs）. The modified electrode have been obtained by layer-by-layer self-assembly methods using dithiothreitol（DTT）, Au@Ag CS NPs, and L-cysteine（L-Cys）. The mechanism of the modified electrode for recognition of melamine molecule has been discussed with electrochemical impedance and cyclic voltammetry behaviors. The impedance value of the modified electrode was linear to logarithmic concentration of melamine in range of 1.0 × 10^-7-1.0 × 10^-2mol/L, and the corresponding linear regression equation is y=1 049.7＋56.75x. The correlation coefficient is 0.997 6 and the detection limit is 6.553 × 10^-8mol/L. The proposed electrode possessed nice selectivity and reproducibility, and can be well applied to the determination of melamine in milk samples with a recovery rate of 98.2%-106.0%, showing its potential application prospect.
出处
《化学传感器》
CAS
2013年第2期38-44,共7页
Chemical Sensors
基金
国家自然科学基金(21275022
21075011
21105005)
湖南省教育厅科研基金(12B002)
国家科技支撑计划(2012BAC17B01)课题资助
