摘要
人工重组的白细胞介素１β（ＩＬ－１β）多次免疫兔和豚鼠，获取高效价的兔抗ＩＬ－１β抗体。用氯氨Ｔ法制备１２５Ｉ标记ＩＬ１β，经Ｓｅｐｈａｄｅｘ Ｇ－２５纯化。该法测定范围０．０６—４ｎｇ／ｍ ｌ，批内和批间误差分别小于５％ 和１０％ 。正常人血清含量为０．２４±０．０８ｎｇ／ｍ ｌ（ｎ＝１３８）。人粒细胞系ＨＬ６０（１０６）细胞体外培养２４ 小时，不能检出上清液中ＩＬ－１β，钙离子载体Ａ２３１８７和磷脂酶Ａ２ 抑制Ｐ－ＢＰＢ也不能显著提高细胞释放ＩＬ－１β。另外，肝癌甲胎蛋白阳性患者血清中ＩＬ－１β水平同正常人没有显著差异。但是，雄性老龄大鼠血清ＩＬ－１β水平明显高于雌性老龄大鼠（Ｐ＜ ０．０１）
Only the high effect antibody of rabbit IL\|1β was obtained by immunizing rabbits and guinea pigs with recombinant IL\|1β many times. The IL\|1β was iodinated with chloramine\|T methods and purified by the Sephadex\|G25 chromatograph column.The determine range of this method was about 0.06—4ng/ml and the lowest detecting level was 0.06ng/ml.The mean errors in group and between groups were less than 5% and 10% respectively.The level of IL\|1β in normal peoples was 0.24±0.08ng/ml(n=138).The liberation of IL\|1β from HL\|60 cells was not obviously promoted by cultured with calcium ionophore(A 23187 ) and phospholipase A\-2 inhibitor (P\|BPB) for 24 hours in vitro.Otherwise,there were no differences in serum IL\|1β level between normal patients and liver cancer patients who was positive in serum alphafetoprotein.Whereas,the IL\|1β in serum of aged male rats was significantly higher than that of aged female rats(P<0.01).\;
出处
《生物工程进展》
CSCD
1999年第6期68-70,共3页
Progress in Biotechnology
