摘要
用人工重组的白介素 6 (IL 6 )多次免疫兔和豚鼠 ,获取高效价的IL 6抗体 .用氯氨T法制备12 5I标记IL 6 ,经SephadexG 2 5纯化 ,抗原抗体反应采用平衡一步法 ,4℃温育 2 4h后经PR试剂分离结合和游离的标记抗原 .该法测定范围 0 1～ 3 2 μg/L最低检出量为 0 lμg/L ,批内和批间误差分别小于 6 4%和 10 % .健康男性115例血清IL 6含量为 (0 2 7± 0 13) μg/L .女性 10 1例血清IL 6为 (0 2 6± 0 10 ) μg/L .男女无差异 .此外 ,用该法检测兔失血性休克再灌注损伤后 2 4h血清IL 6水平明显升高 .失血性休克大鼠淋巴液中IL 6水平明显上升 ,经山莨菪碱 (lmg/kg)治疗休克后IL 6又明显下降 .内毒素同人牙周纤维细胞共同培养不同时间也促进IL
The high effective antibody of interleukin 6 was obtained by immunizing rabbits and guinea pigs with recombinant IL 6 many times. The IL 6 was labeled by 125 I with chloramines T methods and purified by the Sephadex G25 chromatograph column. The reaction between antigen and antibody was carried out by one step balance method and incubated in 4℃ for 24 hours, then separated bond and free antigen by PR reagent. The detection range of this method was about 0 1～3 2 μg/L, the lowest detection level was 0 1 μg/L, error within batches and between batches was less than 6 4% and 10% respectively. The serum IL 6 concentration in normal male was (0 270±0 13) μg/L ( n =115), and in female was (0 260±0 10) μg/L ( n =101), there was no difference in male and female group. Otherwise, the level of IL 6 in serum of rabbits was significantly higher than that of self control at 24 hours after hemorrhagic shock and reperfusion. IL 6 in lymph fluid of rat after hemorrhagic shock was significantly elevated, then it was descended by the treatment of anisodamime (1 mg/kg). The liberation of IL 6 also was promoted and obviously higher than that of the control when fibre cells around tooth were cultured with endotoxin (10 mg/L) at different time in vitro .
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第5期541-544,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金!(39770 716 )
