摘要
目的 :研究肠缺血 -再灌注损伤对外周血及中枢组织瘦素 (Leptin)及Orexin -A蛋白质水平的影响 ,探讨Leptin及Orexin -A在急性炎症反应中的作用。方法 :建立大鼠肠缺血 -再灌注损伤模型 ,实验大鼠随机分为六组 :假手术组 (sham)、缺血 6 0min -再灌注 30min组 (I6 0′R30′)、I6 0′R90′、I6 0′R15 0′、I6 0′R2 4 0′及I6 0′R36 0′组 ,每组 9只。采用放射免疫分析法测量血清及脂肪组织Leptin、血浆及下丘脑组织Orexin -A浓度的变化。结果 :与损伤前自身对照组的血清Leptin水平相比 ,I6 0′R30′组显著降低 ,I6 0′R36 0′组显著增高 ;与损伤后sham组的血清Leptin水平相比 ,I6 0′R36 0′组显著增高 ;与损伤后sham组的脂肪组织Leptin水平相比 ,I6 0′R30′、I6 0′R90′组显著降低 ,I6 0′R36 0′组显著增高。与损伤前自身对照组的血浆Orexin -A水平相比 ,各组损伤后的血浆水平与之均无显著性差异 ;与损伤后sham组血浆或下丘脑Orexin -A水平相比 ,其他各组损伤后的血浆或下丘脑Orexin -A水平与之的差异均无显著性。结论 :Leptin及Orexin -A对肠缺血 -再灌注损伤的应答具有时间依赖效应 ,并在急性炎症反应的能量代谢障碍中发挥一定作用。
Objective To investigate the changes of blood and tissue contents of leptin and orexin-A in rat models of intestinal ischemia-reperfusion(IR) injury for different legnth of time and to explore their possible role in acute inflammatory reaction. Methods Rat models of intestinal ischemia-reperfusion injury were prepared by operative clamping of the superior mesenteric antery for 60min. followed by removal of the clamp and reperfusion for 30, 90, 150, 240 and 360min. (9 animals in each group) before sacrifice. Serum and adipose tissue contents of leptin as well as plasma and hypothalamus contents of orexin-A were determined with a method of RIA developed in this laboratory in these animals both before the injury as self-control value (for blood contents only) and after sacrifice. A group of 9 animals underwent sham operation (laparatomy only) and contents of leptin and orexin-A were tested in the same way. Results Serum levels of leptin decreased significantly in the 30min, reperfusion group (vs preinjury selfcontrol value, P<0.05), but increased significantly in the 360min, reperfusion group (vs self-control, P<0.05). Significant changes of leptin contents were also observed under the following conditions: ① Significantly increased serum contents in the R 360min group (vs sham group, P<0.05), ② Significantly decreased adipose tissue contents in R 30 and R 90 groups (vs sham, P<0.05) and ③ Significantly increased adipose tissue contents in R 360 group (vs sham, P<0.05). However, the theoretically predicted immediate increase followed by grudual decrease thereafter of the orexin-A contents in the reperfusion study was not substantiated and no significant changes of plasma and hypothalamus contents of orexin-A were observed at all. Conclusion A time-dependent response of leptin to ischemia-reperfusion injury was observed in the present experiment. For orexin-A, the response was not evident, possibly masked by the influence of anasthesia. Future modification of the design of experiment might be desirable. Precise role of both factors in the acute inflammatory reaction remains to be clarified.
出处
《放射免疫学杂志》
CAS
2004年第5期329-332,共4页
Journal of Radioimmanology
基金
国家自然科学基金 (No .3 9970 717)
