摘要
对1株产纤溶酶毕赤酵母工程菌菌株pk53,利用单因素试验和正交试验确定该菌株的适宜廉价发酵培养基为:麸皮1.5%,豆粕粉2.0%,KH2PO40.5%,MgSO4.7H2O 0.05%;发酵条件为:接种龄14 h,接种量1%,甲醇添加量1.5%,培养基装液量30 mL(250 mL三角瓶),生长pH为5.5,诱导pH为6.0;在此条件下培养,纤溶酶酶活力达429.06 U/mL,是初始发酵条件下酶活力的8.88倍。
The fermentation medium and conditions of the strain were studied via single factor test and orthogonal design. The optimal fermentation medium was determined as follows： glucose 1. 5%, soypeptone 2. 0%, KH2PO4 0.5% , MgSO4 · 7H2O 0.05 %. The best fermentation conditions were ： inoculating time 14 h, inoculating amount 1% , methanol supply 1.5 % , medium volume 30 mL in 250 mL flask,initial pH 5.5. Under such conditions,the activity of fibrinolytic enzyme （corresponding to the unit of urokinase activity） was 429.06 U/mL,whieh was 8.88 times of the initial strain.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第3期94-98,共5页
Food and Fermentation Industries
基金
河北省十一五农产品加工富民工程专项课题:农产品加工用高效微生物菌种培育及菌种资源库建设(06220106D)
关键词
毕赤酵母
纤溶酶
发酵
优化
Pichia pastoris,fibrinolytie enzyme,fermentation,optimization
