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培养条件对甲醇毕赤酵母异源表达木质素过氧化物酶的影响 被引量：5

Effect of Cultivation Condition on Heterologous Expression of Lignin Peroxidase in Pichia methanolica
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摘要 对含有黄孢原毛平革菌木质素过氧化物酶基因(LipH8)的甲醇毕赤酵母培养基和发酵方法进行了研究．摇瓶培养条件研究结果表明:用11°Bx麦芽汁培养工程菌,然后用高温浸提液置换诱导产酶较好．5 L发酵罐产酶放大实验结果表明,木质素过氧化物酶较稳定,在72 h木质素过氧化物酶酶活达到最高7 568 U/L． The medium and fermentation method of Pichia methanolica with lignin peroxidase gene （LipH8） of Phaneroehaete chrysosporium was investigated. The optimum fermentation method of lignin peroxidase was studied by shake-flask culture. It is good that Pichia methanolica was cultured in the 11°Bx wort medium and then induced in the high temperature malt extract medium. In 5 L fermenter the lignin peroxidase activity was stable and reached the highest activity of 7568 U/L.
作者 王海宽 路福平
机构地区 天津市工业微生物重点实验室
出处 《天津科技大学学报》 CAS 2007年第1期33-36,共4页 Journal of Tianjin University of Science & Technology
关键词 异源表达 木质素过氧化物酶 甲醇毕赤酵母 黄孢原毛平革菌 heterologousexpression ligninperoxidase Pichiamethanolica Phanerochaetechrysosporium
分类号 TQ92 [轻工技术与工程—发酵工程]
  • 相关文献

参考文献7

  • 1[1]Haikuan Wang,Fuping Lu,Yafan Sun,et al.Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica[J].Biotechnol Lett,2004,26:1569-1573.
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  • 4[4]Mohamed S A,Sami S,Ali G.Heterologous expression of lignin peroxiedase of Phanerochaete chrysosporium in Aspergillus niger[J].Biotechnol Lett,1999,21:849-853.
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  • 6王海宽,孙亚范,杜连祥,路福平.木质素过氧化物酶基因(LipH8)的克隆及在甲醇毕赤酵母中的表达[J].微生物学报,2004,44(2):258-260. 被引量：12
  • 7[7]Tien M,Kirk T K.Lignin peroxidase of Phanerochaete chrysosporium[J].Methods Enzymol,1988,161:238-249.

二级参考文献27

  • 1李越中,高培基,王祖农.黄孢原毛平革菌合成木素过氧化物酶的营养调控[J].微生物学报,1994,34(1):29-36. 被引量：51
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  • 7Ludovic O, Eric R, Sonia L, et al. Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus 1 - 937 and expression in Pichia pastoris . Eur J B iochem , 2000, 267:1619-1625
  • 8Mohamed S A, Sami S, Ali G. Heterologous expression of lignin peroxiedase of Phanerochaete chrysosporium in Aspergillus niger.Biotechnology Letters , 1999,21 : 849 - 853.
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共引文献11

同被引文献44

引证文献5

二级引证文献11

天津科技大学学报
2007年 第1期

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