摘要
目的:提取南瓜苗总黄酮并研究其抗氧化活性。方法:用乙醇提取南瓜苗总黄酮,采用DPPH法、水杨酸法检测其对DPPH自由基和.OH的清除作用。结果:最佳提取条件为提取温度70℃、提取时间4h、乙醇体积分数85%、料液比1:25(g/mL)。在该条件下南瓜苗总黄酮得率最高,达到2.56%。南瓜苗总黄酮清除DPPH自由基和.OH的EC50(半抑制质量浓度)分别为0.017mg/mL和0.019mg/mL。结论:南瓜苗中含有丰富的黄酮,并且其具有较强的清除自由基能力。
Aim：To optimize the extraction of total flavonoids from pumpkin seedlings and to evaluate their free radical scavenging activity.Methods：Aqueous ethanol solution was the solvent for flavonoid compounds in pumpkin seedlings. Orthogonal array design method was employed for the optimization of extraction process.Free radical scavenging activity evaluation was achieved by determining the abilities to scavenge DPPH and hydroxyl free radicals（by salicylic acid method）. Results：The optimized extraction conditions were found to be extraction at 70℃for 4 h using 85%aqueous ethanol solution at a solid/liquid ratio of 1：25（g/mL）.The extraction efficiency for total flavonoids from pumpkin seedlings reached up to 2.56% under these conditions.The median effective concentration（EC50）of total flavonoids from pumpkin seedlings against DPPH and hydroxyl free radicals were determined to be 0.017 mg/mL and 0.019 mg/mL,respectively.Conclusion：Flavonoid compounds are abundant in pumpkin seedlings and those from pumpkin seedlings have strong free radical scavenging activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第8期58-60,共3页
Food Science
基金
广东省科技计划项目(2008A024200003)
关键词
南瓜苗
总黄酮
提取
抗氧化活性
pumpkin sprout
total flavonoids
extraction
antioxidant activity
