摘要
[目的]为忍冬种质资源的合理利用提供理论依据。[方法]运用RAMP标记技术对8个忍冬品种的遗传多样性进行分析,并采用UPGMA方法对其进行聚类。[结果]8个忍冬品种均具有较丰富的遗传多样性,其多态性比率为83.67%,遗传相似系数为0.5901～0.971 3,平均值为0.784 1。Nei's遗传距离和UPGMA分析结果显示,8个忍冬品种被聚为2大类,其中淡红忍冬与金银花的遗传相似系数最小(0.590 1),亲缘关系最远;贯月忍冬与格雷姆忍冬的遗传相似系数最大(0.971 3),亲缘关系最近。[结论]应用RAMP标记可有效分析忍冬品种的遗传多样性,为忍冬种质资源的鉴别、分类和有效利用提供依据。
[Objective] The aim was to supply theoretical basis for reasonable utilization of honeysuckle germplasm resource.[Method] The genetic diversity of 8 honeysuckle varieties was analyzed by applying RAMP labeling technique and they were clustered by UPGMA method.[Result] The 8 honeysuckle varieties all possessed relatively rich genetic diversity,their polymorphism ratio,genetic similarity coefficients and average genetic similarity coefficient were 83.67%,0.590 1-0.971 3 and 0.784 1 resp.The results from Nei＇s genetic distance and UPGMA analysis showed that the 8 honeysuckle varieties were clustered to be 2 main categories,the genetic similarity coefficient between Lonicera henryi Hemsl and Lonicera japonica was smallest,being 0.590 1 and their kinship was farthest;the genetic similarity coefficient between Lonicera sempervirens and Lonicera periclymenum was biggest,being 0.971 3 and their kinship was nearest.[Conclusion] RAMP labeling technique was available to analyze the genetic diversity of honeysuckle varieties and this technique supplied foundation for identification,classification and available utilization of honeysuckle germplasm resource.
出处
《安徽农业科学》
CAS
北大核心
2011年第9期5098-5100,共3页
Journal of Anhui Agricultural Sciences
基金
林业公益性行业科研专项(201104039)资助
关键词
RAMP分子标记
忍冬
品种鉴定
遗传多样性
RAMP molecular marker
Honeysuckle
Variety identification
Genetic diversity
