摘要
对仲彬草属Kengyilia 14个种和1个变种进行了RAMP分析。结果表明物种间遗传差异明显。40个引物组合产生的254条DNA扩增片段中,216条(85.0％)具有多态性,每个引物组合可扩增出1～9条多态性带,平均5.4条。遗传相似系数变化范围为0.327～0.886,平均值为0.549。同时,形态相似、地理分布一致的物种有一定的亲缘关系,聚类在一起。这与RAPD、形态学和细胞学等分析结果基本一致。因此,RAMP分子标记是评价仲彬草属种间关系十分有效的方法。
Relationships among 14 Kengyilia species and 1 variety were analyzed using random amplified microsatellite polymorphism (RAMP) markers. The results showed distinct genetic differences were present among the species. 40 primer combinations produced 254 bands, among which 216 out of 254 bands (85.0%) were polymorphic. 1-9 polymorphic bands could be amplified from each primer combination, with an average of 5.4 bands. The genetic similarity coefficients ranged from 0.327 to 0.886, with the mean of 0.549. Meantime, the species with similar morphological characters and the species from the same areas or neighboring geographical regions were clustered together, which suggested that had close relationships. RAMP results are basically comparable with those obtained from studies on RAPD, morphology and cytology. It is a useful method for analysis of the relationship in Kengyilia.
出处
《高技术通讯》
EI
CAS
CSCD
2003年第4期28-33,共6页
Chinese High Technology Letters
基金
国家自然科学基金(30270099)
四川省教育厅和科技厅重点基金资助项目。
