摘要
对24份优质抗稻瘟病水稻种质资源进行了RAMP（random amplified microsatellite polymorphism）标记分析。结果表明，80个RAMP引物组合中，有22个引物组合可扩增出清晰且具多态性的条带。这22个引物组合共扩增出108条带，其中80条具有多态性，占74．1％，每个引物组合可扩增出1～9条多态性带，平均3．6条。RAMP标记遗传相似性系数（GS）平均值为0．462（0．138～0．900）以平均GS值0．462为阈值，可将所有供试材料划分为6类。表明这24份优质抗稻瘟病种质资源具有较丰富的遗传多样性。这些优质抗源材料对选育高产、优质、抗稻瘟病新品种（组合）具有重要利用价值。
The genetic diversity of 24 good quality rice germplasm resources resistant to rice blast was evaluated by using random amplified microsatellite polymorphism （RAMP） markers. The results showed that 22 out of 80 RAMP primers, which could amplify polymorphic bands dearly, were selected for the PCR amplification of genomic DNA. A total of 108 bands were amplified from 22 primers, while 80 bands （about 74.1%） were polymorphic. One to 9 polymorphic bands could be amplified from each primer, with an average of 3.6 bands. The RAMP-based genetic similarity （GS） among 24 rice germplasm resources ranged from 0.138 to 0.900. Based on the GS matrix, a dendrogram was constructed by using the unweighted pair group method with arithmetic average （UPDMA）. All the 24 rice germplasm resources could be distinguished by RAMP makers. Clustering analysis showed that the 24 tested resources could be classified into 6 groups at 0. 462 similarity coefficient. It indicated that these 24 rice germplasm resources had highly genetic diversity. It was of important value to breed new high-yield and good quality varieties or hybrid rice combinations resistant to blast.
出处
《四川农业大学学报》
CSCD
2007年第1期24-28,33,共6页
Journal of Sichuan Agricultural University
基金
四川省育种攻关项目
四川省应用基础项目和四川农业大学人才引进项目资助。
关键词
水稻
优质
抗稻瘟病
种质资源
RAMP标记
遗传多样性
rice
good quality
rice blast resistance
germplasm resources
RAPM
genetic diversity
