摘要
目的 通过研究ＴＴ病毒在谷丙转氨酶升高的体检人群和肝病患者中的意义，探讨其致病性。方法 收集１９ 例谷丙转氨酶升高体检者和４１ 例转氨酶正常的随机对照的血清，以及１８２ 例肝病患者的血清，采用ＰＣＲ 方法检测ＴＴ病毒的ＤＮＡ。聚合酶链反应（ＰＣＲ） 产物经限制性片段长度多态性（ＲＦＬＰ） 分析验证。同时检测甲、乙、丙、戊、庚型肝炎病毒（ＨＡＶ、ＨＢＶ、ＨＣＶ、ＨＥＶ和ＨＧＶ） 感染标志。结果 １９ 例转氨酶升高体检者中，ＴＴ病毒ＤＮＡ 检出率为１０－５３％ （２１９），对照组检出率为２－４４％ （１４１） ，两者差异无显著意义（ Ｐ＞０－０５）。转氨酶升高者中，９ 例为ＨＢＶ 现症感染，１ 例为丙型肝炎，１ 例为甲型肝炎。２ 例ＴＴ病毒ＤＮＡ 阳性者均重叠ＨＢＶ 现症感染。肝病患者中ＴＴＶＤＮＡ 检出比率为４－４％ 。１８ 例非甲～庚型肝炎中仅１ 例为ＴＴＶＤＮＡ阳性。结论 在本组健康体检人群中能检出一定水平的ＴＴ病毒感染，但引起体检者转氨酶升高的主要原因为ＨＢＶ感染。在肝病患者中也存在一定水平ＴＴ病毒感染，但不是非甲～庚型肝炎的主要病因。
Objective To investigate the significance of the new DNA virus (TTV) infection in persons with elevated alanine aminotransferase (ALT) during routine medical check up and patients with liver diseases, and analyze its pathogenesis.Methods PCR was used in the detection of TTV DNA in 19 serum samples from ALT elevated persons and 41 from ALT normal persons during their routine medical check up, and 182 serum samples from patients with various kinds of liver diseases. PCR products were confirmed by RFLP. Other hepatitis viruses were simultaneously detected using routine methods. Results TTV DNA was detected in 10.53%(2/19) and 2.44%(1/41) in groups of ALT elevated and control respectively with no significant difference between their detective rates ( P >0.05). There were 9 cases with current HBV infection, 1 case with hepatitis C and 1 case with hepatitis A in group of elevated ALT. Two TTV positive cases were both superinfected with HBV. The detecting rate of TTV DNA in patients with liver diseases was 4.4%. Only one case out of 18 non A to G hepatitis showed TTV DNA in serum. Conclusion There were TTV infections in Chinese population. The main cause for ALT elevation in persons during routine medical check up was HBV infection. There were TTV infections in patients with liver diseases too. However, TTV wasn′t the main cause of non A to G hepatitis. Thus, the pathogenesis of TTV might be very weak.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1999年第3期276-279,共4页
Chinese Journal of Experimental and Clinical Virology
基金
广东省自然科学基金
中山医科大学"211 工程"专项基金
关键词
病毒性肝炎
转氨酶
TT病毒
病原学
Viral hepatitis
Alanine aminotransferase
TT virus
Etiology
Pathogenesis
