摘要
通过刚果红染色法和DNS分光光度法对6种芽胞杆菌分泌胞外纤维素酶进行筛选,再通过管碟法测试对5种病原菌的抑菌作用,得到1株芽胞杆菌(菌株编号为X-02)酶活达182.5 U/mL,而且对几种土传病害有抑制作用。并对其产酶发酵培养基碳源、氮源及初始pH、发酵温度、接种量、摇瓶转速和时间进行优化,结果显示该菌株最佳碳源是2%CMC-Na,其次是葡萄糖,二者产酶之差只为21 U/mL。考虑大量生产的成本和方便性(CMC-Na溶解慢),选择葡萄糖为碳源,氮源为2.0%蛋白胨与酵母膏复合氮源,在pH值为8.0、温度37℃、接种量为2%、转速为180 r/m in、时间48 h条件下酶活达到391.0 U/mL酶液,比优化前提高了2.1倍。
A Bacillus strain（serial number X-02） was screened among six kinds of Bacillus that excrete extracellular cellulase were screened through DNS spectrophotometer and the Congo red staining,by testing with 5 kinds of pathogen for their inhibition with cup-plate method;and the enzyme activity was as high as 182.5 U/mL and had the inhibition against several soil borne pathogens.Later carbon source,nitrogen source and initial pH of its fermentation medium,temperature,inoculation quantity,rotation of shake flask and time were optimized.The results showed that the best carbon source was 2% CMC-Na,2.0%,the next was glucose,and the difference of the enzyme output was only 21 U/ml,considering the production cost and convenience for large-scale production（slow solubilization of CMC-Na） glucose was chosen as carbon source,and nitrogen source was a compound one of peptone and yeast extract.The enzyme activity was as high as 391.0 U/mL and was 2.1 times as compared with the one before the optimization under the conditions of the pH 8.0,37 ℃,2% inoculation,180 rpm for 48 h.
出处
《微生物学杂志》
CAS
CSCD
2011年第1期63-67,共5页
Journal of Microbiology
基金
国家农业科技成果转化项目(国科发农[2010]297号)
辽宁省科技厅攻关计划项目(2009301005)
关键词
纤维素酶
芽胞杆菌
发酵
管碟法
病原菌
cellulase
Bacillus
fermentation
cup-plate method
pathogen
