摘要
目的:建立测定紫草中β,β′-二甲基丙烯酰阿卡宁含量的方法。方法:采用改进后的高效液相色谱法,以流动相制备供试品溶液。色谱柱为Kromasil100-5C18(150mm×4.6mm,5μm),流动相为乙腈-水-甲酸(700∶300∶0.5),流速为1.0mL·min-1,检测波长为275nm。结果:β,β′-二甲基丙烯酰阿卡宁的进样量在0.16～3.20μg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为99.46%,RSD=1.90%(n=6)。结论:该方法简便、易行,方法验证符合要求,可用于紫草的含量测定。
OBJECTIVE：To establish a method for the content determination of β,β＇-dimethylacrylalkannin in Radix Arnebiae.METHODS：Improved HPLC method was adopted and samples were prepared by mobile phase.Kromasil 100-5 C18（150 mm×4.6 mm,5 μm） column was used with mobile phase consisted of acetonitrile-water-formic acid（700：300：0.5）at flow rate of 1.0 mL· min-1.The detection wavelength was set at 275 nm.RESULTS：β,β＇-dimethylacrylalkannin had good linearity in the range of 0.16-3.20 μg（r=0.999 9） with an average recovery of 99.46%（RSD=1.90%,n=6）.CONCLUSION：The method is simple and feasible for content determination of Radix Arnebiae.Method verification is up to the standard.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第15期1407-1408,共2页
China Pharmacy
